体外联合药敏试验分析氯氰碘柳胺与环丙沙星对肠球菌的抗菌作用  

Antibiotic effect of closantel and ciprofloxacin on entercoccus spp. by combinational susceptibility test in vitro

作　　者：祝忠良[1] 吕火祥[1] 顾丽芳 胡利春[1] 

机构地区：[1]浙江省人民医院检验中心,杭州310014

出　　处：《中国卫生检验杂志》2012年第5期1028-1030,共3页Chinese Journal of Health Laboratory Technology

基　　金：浙江省自然基金资助项目(Y2100445)

摘　　要：目的:评价氯氰碘柳胺(closantel)与环丙沙星联用对多重耐药的肠球菌的体外联合抗菌效应。方法:采用棋盘法和微量肉汤稀释法,测定不同浓度组合的抗菌药物对40株肠球菌的最低抑菌浓度(MIC),计算出抗菌药物的FIC指数来判定联合抗菌效应。结果:氯氰碘柳胺与环丙沙星体外联合用药,其FIC指数分布:FIC≤0.5占30%;0.5<FIC≤1占60%;1<FIC≤2占10%;FIC>2占0。其中粪肠球菌的联合药敏结果 FIC≤0.5的占50%,0.5<FIC≤1的占40%;屎肠球菌的联合药敏结果 0.5<FIC≤1的占80%。结论:氯氰碘柳胺与环丙沙星体外联合药敏对肠球菌主要为协同和相加作用。粪肠球菌效果优于屎肠球菌。Objective：To evaluate the combination susceptibility testing of closantel and ciprofloxacin for multiple-drug resistant Enterococcus spp.in vitro.Methods： The chessboard method and micro-broth dilution method were used to determine the minimal inhibitory concentration（MIC） of antimicrobial agents with different combinations on 40 strains of enterococcus spp.and calculate the FIC indices to judge combinational antibiotic effect.Results： With combination therapy of closantel and ciprofloxacin,the FIC≤0.5 accounted for 30%,0.5FIC≤1 accounted for 60%,1FIC≤2 accounted for 10%,FIC2 accounted for 0%.The susceptibility test result of enterococcus spp.showed that FIC≤0.5 accounted for 50%,0.5FIC≤1 accounted for 40%,while that of entercoccus faecalis showed 0.5FIC≤1 accounted for 80%.Conclusion： The combinational antibacterial effect of closantel and ciprofloxacin for Enterococcus spp.in vitro were mainly collaboration and add function.And,the effect for Enterococcus faecalis is better than that of Enterococcus faecium.

关 键 词：肠球菌 氯氰碘柳胺 环丙沙星 体外联合药敏 

分 类 号：R378.1[医药卫生—病原生物学]