中国棉铃虫核多角体病毒(Heliothis armigera NPV)VHA273毒株DNA的酶切分析与比较研究  

The endonudease analysis and comparative studies of the VHA273 strain DNA of Heliothis armigera NPV

作　　者：吴柏春[1] 

机构地区：[1]华中师范大学,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2000年第1期74-77,共4页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金!84030313

摘　　要：VHA273毒株原为MNPV型，经纯系中国棉铃虫扩增而得出约10％的SNPV.高度纯化两型多用体，温和碱解后，酚法抽提SNPVDNA和MNPVDNA.限制性内切酶BglⅡ，BamHI，EcoRI和HindⅢ分别平行酶切两种DNA，依次均得出11，8，14和13条电泳带．比较分析DNA的酶切电泳图谱发现，经同一种限制性内切酶酶切，SNPVDNA与MNPVDNA不仅电泳片段数相同，电泳带位也-一对应．讨论提出：该SNPVDNA与MNPVDNA在分子、亚分子水平上是一致的．The SNPV were produced from the cadavers of purifine line Heliothis armigera infected with VHA273 strain (MNPV) of Heliothis armigera nuclear polyedrosis virus. The polyhedra of the SNPV and the MNPV were highly purified and gentley degraded with base respectively. The SNPV DNA and the MNPV DNA were extracted by water-saturated phenol by means of two-step method. The DNAs appeared to be characteristics of DNA in ultraviolet absorption spectrum. Restriction endonuclease analysis of the SNPV DNA and the MNPV DNA agarose gel electrophoresis revealed that Bgl Ⅱ, BamHI, EcoRl and Hind Ⅲ cleaved the DNAs into 11, 8, 14 and 13 fragments respectively. Comparative studies of the restriction endonuclease patterns showed that the DNAs fragment attained from the SNPV DNA and the MNPV DNA by the same endonuclease was of the same numbers and corresponded with one another in position in sagaros gel respectively. This paper suggested the SNPV DNA and the MNPV DNA of the VHA273 strain of the Heliothis armigera nuclear polyhedrosis virus were identical in molecular and submolecular levels.

关 键 词：酶切图谱 VHA273毒株 DNA 棉铃虫 核多角体病毒 

分 类 号：TQ453.5[化学工程—农药化工] Q78[生物学—分子生物学]