导数荧光法测定复方新诺明中的磺胺甲胺异噁唑和甲氧苄胺嘧啶  

Derivative Flouorimetric Method for the Determination of Sulfame thoxizole and Trimethoprim in Sulfamethoxizole Composite

作　　者：高建华[1] 王国富[2] 樊卫华[1] 毛陆原[1] 

机构地区：[1]郑州大学化学系,河南郑州450052 [2]中国科技大学化学系,安徽合肥230026

出　　处：《郑州大学学报（自然科学版）》2000年第1期77-79,92,共4页Journal of Zhengzhou University (Natural Science)

基　　金：河南省教委自然科学基金资助项目

摘　　要：在磺胺甲基异口恶唑的激发峰 2 6 9.4nm处记录一阶导数发射光谱 ,采用峰 -谷法测量导数信号 ,在甲氧苄胺嘧啶存在下 ,不经分离和掩蔽实现磺胺甲基异口恶唑的测定 ;用二阶导数激发光谱 ,在磺胺甲基异口恶唑存在下 ,实现甲氧苄胺嘧啶的测定 .磺胺甲基异口恶唑的线性范围是 0～ 4.0 μg/ m L,甲氧苄胺嘧啶的线性范围是 0～ 3.0 μg/ m L.A derivative fluorimetric method was developed for the determination of trimethoprim and sulfamethoxizole. First derivative emission spectra were recorded at 269.4nm which was the exitation wavelength of sulfamethoxizole, sulfamethoxizole was determined in the presence of trimethoprim by measuring peak valley signal. Second derivative exitation spectra was recorded at the emission wavelength, 341.5nm. Trimethoprim was directly determined without any seperation and masking, the determination signal being measured by zero crossed intercept method. The linear relationship was obeyed in the range of 0 4.0μg/mL for sulfamethoxizole and 0-3.0μg/mL for trimethoprim. The instrument conditions were also selected. The method was simple, rapid and well repeated, and had been applied to the determ ination of trimethoprim and sulfamethoxizole in sulfamethoxizole composite with satisfactory results.

关 键 词：导数荧光法 磺胺甲基异恶唑 复方新诺明 TMP 

分 类 号：R978.2[医药卫生—药品] O657.31[医药卫生—药学]