两种全基因组扩增技术对单根毛发DNA检验效能的研究  

The value of two whole genome amplification protocols for single shed hair genotyping

作　　者：邓建强[1] 刘宝琴 蔡继峰[3] 李文慧[1] 龙仁[1] 侯一平[4] 

机构地区：[1]海南医学院法医鉴定中心,海南海口570102 [2]西安现代妇产医院,陕西西安721000 [3]中南大学湘雅医学院法医学系,湖南长沙410078 [4]四川大学华西基础与法医学院,四川成都610041

出　　处：《中国热带医学》2012年第4期389-392,共4页China Tropical Medicine

基　　金：国家自然科学基金资助项目(No.30271446);高校博士点专项科研基金资助项目(No.20020610044)

摘　　要：目的研究简并寡核苷酸引物PCR(degenerate oligonucleotide-primed PCR,DOP-PCR)和扩增前引物延伸PCR(primer extension pre-amplification PCR,PEP-PCR)对单根毛发检验的法医学价值。方法以三种方法对单根毛发样本进行STR分型,第一种方法直接对提取DNA用Profiler Plus试剂盒进行STR分型,第二种和第三种方法是先对提取的DNA样本分别进行DOP-PCR和PEP-PCR扩增处理,然后对其产物用Profiler Plus试剂盒进行STR分型,对两种方法的效能进行比较评价。结果 DOP-PCR和PEP-PCR技术增加了单根毛发STR分型的准确率和信息量。结论 DOP-PCR和PEP-PCR技术对单根自然脱落毛发的检验具有实用价值。Objective To estimate the practical value of degenerate oligonucleotide-primed PCR（D0P-PCR）and primer extension pre-amplification PCR（PEP-PCR）protocols in genotyping of single shed hair.Methods DNA samples obtained from single shed hair were genotyped by three ways： the first one was that the DNA saples were genotyped directly by Profiler Plus kit;the second and thord ones were that the original DNA samples were amplified by DOP-PCR and PEP-PCR protocol respectively,then their products were genotyped by Profiler Plus kits as first way.The results of three ways were compared.Results DOP-PCR and PEP-PCR protocols improved accuracy and informamtion for single shed hair genotyping.Conclusions DOP-PCR and PEP-PCR protocol have practical values for STR genotyping of single hair samples.

关 键 词：简并寡核苷酸引物 扩增前引物延伸 法医学 毛发检验 

分 类 号：R446.7[医药卫生—诊断学]