海洋细菌的分离、鉴定及抗烟草花叶病毒活性分析  

Isolation,identification and antiviral activity of marine bacteria against Tobacco mosaic virus

作　　者：韩凤英[1,2] 谢荔岩[1] 连玲丽[1,3] 林奇英[1] 

机构地区：[1]福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福建福州350002 [2]山东省农业管理干部学院,山东济南250100 [3]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2012年第3期281-285,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省青年人才项目(2008F3011);福建省发改委资助项目(2002153)

摘　　要：从福建霞浦海域分离、筛选到6株对烟草花叶病毒(TMV)具有体外钝化活性的菌株,其中,菌株9和10的抑制率较高,分别达95.26%和92.18%.进一步测定2株菌对TMV的抑制增殖活性,结果表明,菌株9和10可明显抑制TMV的增殖,其抑制率分别为36.29%和35.08%.通过对2株菌的形态特征、生理生化反应及16S rDNA序列的分析,将菌株9鉴定为蜡样芽孢杆菌,菌株10鉴定为水生海洋芽孢杆菌.Six marine bacteria isolated from tidal marine mud in Xiapu, Fujian, exhibited activities against Tobacco mosaic virus （TMV）. Among them, the strain 9 and 10 showed strong antiviral activities in vitro, resulting in a inhibition rate of 95.26% and 92.18% respectively. Further tests on their antiviral activities in vivo indicated that both strains inhibited the reproduction of TMV particle and the inhibition rates were 36.29% and 35.08% respectively. According to the morphological physiological characters and 16$ rDNA sequence, strain 9 was identified as Bacillus cereus, strain 10 Was identified as Bacillus aquimaris.

关 键 词：海洋细菌 抗烟草花叶病毒活性 分离鉴定 

分 类 号：S432.41[农业科学—植物病理学]