尿激酶对急性深静脉血栓大鼠受累血管管壁的保护作用  

Protective effect of urokinase on vascular walls of the affected vessels in rats with deep vein thrombosis

作　　者：王护国[1] 哈尔满·阿吉汗[1] 赛力克·马高维亚[1] 罗军[1] 

机构地区：[1]新疆医科大学第一附属医院血管甲状腺外科,新疆乌鲁木齐830011

出　　处：《中国普通外科杂志》2012年第6期687-692,共6页China Journal of General Surgery

摘　　要：目的:观察不同剂量尿激酶对急性深静脉血栓形成大鼠不同时期治疗后受累血管管壁的影响。方法:将96只SD大鼠制备急性深静脉血栓模型后,随机按血栓形成后的时间(1,3,7,14 d)分期,均分为:Ⅰ,Ⅱ,Ⅲ,IV期;每期再均分为:模型组,尿激酶小剂量组[2万U/(kg.d)],尿激酶中剂量组[4万U/(kg.d)],尿激酶大剂量组[(8万U/(kg.d)]。尿激酶每日以尾静脉注射,连续7 d。实验结束后获取受累段血管,分别通过HE染色和苦味酸-天狼猩红染色观察各组血管内皮细胞数量和胶原含量的变化。结果:在Ⅰ,Ⅱ,Ⅲ期,尿激酶3个剂量组内皮细胞数量均明显高于模型组(均P<0.05),但尿激酶3个剂量组间差异无统计学意义(P>0.05);胶原阳性染色面积百分比均明显低于模型组(均P<0.05),且随着尿激酶剂量的增加而减少。在IV期,尿激酶3个剂量组内皮细胞数量与模型组比较均无统计学的差异(均P>0.05);但尿激酶3个剂量组胶原阳性染色面积百分比仍明显低于模型组(均P<0.05)。结论:在血栓形成14 d内,尿激酶对受累血管的内皮细胞有保护作用,对胶原增生均有抑制作用。在血栓形成14 d后,尿激酶对内皮细胞无保护作用,但对胶原增生仍有抑制作用。Objective： To observe the effects of different doses of urokinase, given at different times after thrombus formation, on the vascular wall of the affected vessels in rats with deep vein thrombosis （DVT）. Methods： Ninety-six SD rats were equally randomized into stage I, stage II, stage III and stage IV groups according to the time elapsed since thrombus formation （1, 3, 7, and 14 d） after the establishment of DVT model. The rats of each stage group were equally randomized again into model group, low-dose urokinase [20 000 U/（kg.d）] group, mid-dose [40 000 U/（kg.d）] urokinase group and high-dose [80 000 U/（kg.d）] urokinase group. Urokinase was administered by tail vein injection for 7 days. Xhe affected vessels of the rats were harvested at the end of the experiment, and the alterations of endothelial cell number and collagen amount were measured via H＆E staining and picrosirius red staining, respectively.Results： In stages I, II and III, the numbers of endothelial cells of the three urokinase treated groups were all signifcantly higher than that of model group （all P〈0.05）, but no statistical significances were noted between the urokinase groups （P〉0.05）. ~he percentages of the collagen-positive staining area of the three urokinase treated groups were all significantly lower than that of model group （all P〈0.05）, and this percentage was reduced with the increasing dose of urokinase. In stage IV, the numbers of endothelial cells of all of the three urokinase treated groups had no significant differences compared with model group （all P〉0.05）, while the percentages of the collagen-positive staining area of the three urokinase treated groups were all still significantly lower than that of model group （all P〈0.05）. Conclusion： Urokinase has significant protective effect on the endothelial cells and inhibitory effect on the collagen proliferation of the affected vessels within the first 14 days after thrombus formation. However, beyond 14 days after thrombus f

关 键 词：静脉血栓形成 尿纤溶酶原激活物 内皮细胞 胶原 模型 动物 

分 类 号：R654.3[医药卫生—外科学]