三丁基锡对褐菖鮋精巢性激素受体表达的影响  

Effect of Tributyltin on the Sex Hormone Receptor in Testis of Sebastiscus Marmoratus

作　　者：张纪亮[1] 朱文文[1] 孙平[1] 熊建利[1] 郭黛健[2] 

机构地区：[1]河南科技大学动物科技学院,河南洛阳471003 [2]洛阳师范学院,河南洛阳471022

出　　处：《海洋科学进展》2012年第2期252-256,共5页Advances in Marine Science

基　　金：国家自然科学基金--有机锡污染对海洋鱼类性腺分化和发育的影响及其机制(20777061);河南省教育厅科技重点研究项目--有机锡水体污染对鱼类性腺发育的影响及其机制(12B240001)

摘　　要：三丁基锡(Tributyltin,TBT)能够影响鱼类生殖功能,而有关TBT对鱼类生殖细胞发育至关重要的雄激素和雌激素受体的影响还未见报道。本实验以海洋经济鱼类褐菖鲉(Sebastiscus marmoratus)为对象,研究环境水平TBT(1,10和100ng/L)对精巢雄激素和雌激素受体表达的影响。结果表明,TBT暴露能够抑制褐菖鲉精巢发育和精巢雌激素受体蛋白的水平,并且发现雌激素受体主要定位于精巢精小叶腔间隙。小叶腔间隙的雌激素受体蛋白水平降低可能影响支持细胞和间隙细胞的正常功能,从而影响精巢的正常发育。TBT暴露对雄激素受体的表达并没有显著的影响。该结果为全面了解TBT产生鱼类生殖毒性效应、途径和机制提供了新的重要的资料。Tributyltin （TBT） can affect the reproductive function in fishes. However, few study reports the effects of TBT on estrogen receptor or androgen receptor which is related to the development of germ cells. Therefore, the present study was conducted to investigate the effects of TPT at environmentally relevant concentrations （1, 10 and 100 ng/L） on testieular estrogen receptor or androgen receptor in male rockfish Sebastiscus marrnoratus. The results showed that TBT inhibited the testicular development and reduced the levels of estrogen receptor which was located in the interlobular septa. TBT interfered with estrogen receptor might be one of the reasons causing the dysfunction of Sertoli cells, which play a critical role during spermatogenesis. Androgen receptor levels were not shown significant changes after TBT ex posure. The results provided new information which is important for fully understanding the reproductive toxic effects, pathway and mechanism of TBT.

关 键 词：三丁基锡 褐菖鮋 精巢 雌激素受体 

分 类 号：Q958[生物学—动物学]