慢性烟曲霉暴露对支气管哮喘大鼠气道黏蛋白MUC5AC表达的影响  

Effects of chronic Aspergillus fumigams exposure on the expression of mucin 5AC in the airways of asthmatic rats

作　　者：高福生[1] 高艳艳[1] 刘美娟[1] 刘永全[1] 

机构地区：[1]潍坊医学院附属医院呼吸科,山东261031

出　　处：《中华结核和呼吸杂志》2012年第6期423-428,共6页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的探讨慢性烟曲霉暴露对支气管哮喘（简称哮喘）大鼠气道黏蛋白MUC5AC表达的影响。方法将56只雄性Wistar大鼠按随机数字表法分为7组（每组8只）：慢性哮喘（A）组、慢性哮喘＋烟曲霉孢子吸人1周（B）、3周（C）和5周（D）组、慢性哮喘＋生理盐水吸人（E）组、卵清白蛋白（OVA）致敏生理盐水激发（F）组和OVA致敏生理盐水激发＋烟曲霉孢子吸入（G）组。测定各组大鼠基础气道阻力和应用乙酰胆碱后气道阻力变化率，RT—PCR测定肺组织MUC5ACmRNA的表达，免疫组织化学染色测定各组大鼠气道上皮细胞MUC5AC的表达，酶联免疫吸附（ELISA）法测定BALF中IL-13水平，肺组织切片过碘酸雪夫染色（PAS）观察气道上皮杯状细胞增生程度。结果B、C和D组大鼠肺组织MUCSACmRNA（MUCSACmRNA／β-actinmRNA）（分别为1．9±0．4、2．3±0．6、2．9±0．8）、气道上皮细胞MUC5AC表达（A值）（分别为278士58、566±64、8914-80）、BALF上清液IL-13[分别为（96±16）、（136±22）、（197±34）μg/L]及杯状细胞／上皮细胞面积（分别为16％±5％、23％±7％、36％±9％）均高于A、E、F及G组（均P〈0．05）；C和D组大鼠气道阻力变化率（分别为61．91％±5．26％、84．69％±6．38％）均高于A、E、F及G组（均P〈0．05）。B、C和D组哮喘大鼠MUCSAC的A值及MUC5ACMma／β-actinmRNA与气道上皮杯状细胞增生程度（PAS染色区面积／上皮细胞面积）呈正相关（r值分别为0．578和0．614，均P〈0．05），与气道反应性（Raw变化率）呈正相关（r值分别为0．638和0．564，均P〈0．05）。结论慢性烟曲霉暴露可上调哮喘大鼠气道上皮黏蛋白MUC5AC的表达，促进杯状细胞增生，加重气道高反应。Objective To explore the effects of chronic Aspergillus fumigatus （Af） exposure on the expression of mucin SAC（MUC5AC） in the airways of asthmatic rats. Methods Fifty-six male Wistar rats were randomly divided into 8 groups： chronic asthma （group A）, chronic asthma plus Af spores inhalation for 1 week （group B）, 3 weeks （group C） and 5 weeks （group D）, chronic asthma plus saline inhalation for 5 weeks （group E）, OVA-sensitized and- saline-challenged group （group F） and OVA-sensitized and- saline-challenged plus Af spores inhalation for 5 weeks （ group G） （ each n = 8 ）. The airway resistance （Raw） and the change rate of Raw after acetylcholine provocation were detected using a computerized system. The level of MUCSAC mRNA in the lung tissue was measured by RT-PCR, and the expression of MUCSAC in airway epithelial cells were demonstrated by immunohistochemistry. The concentration of IL-13 in BALF was measured by ELISA. The extent of goblet cell hyperplasia was evaluated on periodic acid-Schiff stain （PAS） lung sections. Results In group B, C, and D, the level of MUCSAC mRNA （MUC5AC mRNA/β-actin mRNA） （ 1.9 ±0.4, 2. 3± 0. 6, 2. 9± 0. 8, respectively）, the integrated optical density （value A） of MUC5AC positive stain in airway epithelial cells （278 ±58, 566 ± 64, 891 ± 80, respectively）, the concentration of IL-13 in BALF（μg/L） （96± 16, 136 ±22, 197 ± 34, respectively）, and the ratio of goblet cell area to epithelial cell area （ % ） （ 16± 5,23± 7,36 ± 9, respectively）, were higher than those in group A, E, F and G （ all P 〈 0. 05）. The change rate of Raw （ % ） in group C and D （61.91±5.26 and 84. 69 ±6. 38）were higher than that in group A, E, F and G （all P 〈0. 05）. The level of MUC5AC mRNA and the value A of MUC5AC were positively correlated with the ratio of goblet cell area to epithelial cell area （ r = 0. 578, P 〈 0. 05 ;r = 0. 614, P 〈 0. 05, respectively） and the change rate of Raw （

关 键 词：哮喘 烟曲霉 黏蛋白类 上皮细胞 

分 类 号：R562.25[医药卫生—呼吸系统]