不同载体及靶基因对乙型肝炎病毒DNA疫苗免疫效果的影响  

Effects of DNA vector constructs and different genes on the induction of immune responses by HBV DNA-based vaccine

作　　者：王全楚[1] 周永兴[1] 姚志强 冯志华[1] 

机构地区：[1]中国人民解放军第四军医大学唐都医院传染病科,陕西省西安市710038

出　　处：《世界华人消化杂志》2000年第3期289-291,共3页World Chinese Journal of Digestology

基　　金：国家自然科学基金;No.39770665

摘　　要：目的 探讨不同DNA载体及靶基因对DNA疫苗免疫效果的影响。 方法 用已构建的不同载体HBV S基因疫苗(pCR3.1-S,pcDNA3-S)和HBVC基因疫苗(pCR3.1-C)分别给Balb/c小鼠多点肌肉注射,2wk后追加免疫一次,用ELISA法及MTT法分别检测小鼠血清抗-HBs及抗HBc及脾细胞对HBsAg或HBcAg的特异性增殖反应。 结果 免疫接种2wk后小鼠血清抗-HBs及抗HBc抗体均明显高于对照组,载体pCR3.1的表达效力稍高于pcDNA3,但同一基因不同载体间无显著差异,不同靶基因血清抗体滴度及脾细胞对HBsAg或HBcAg的刺激指数均明显高于对照组,刺激指数pCR3.1-C组明显高于单纯pCR3.1-S注射组。 结论 两种载体均可以诱导较强的体液免疫应答;但同一基因不同载体间无显著差异,HBVS和C基因疫苗均可诱导较强的体液和细胞免疫应答强度;C基因以细胞免疫增高为主。AIM To study the effects of DNA vector constructs and different target genes on the efficiency of induction of immune responses to the HBV surface antigen (HBsAg) and HBV core antigen ( HBcAg) in mice by DNA immunization.METHODS The HBV surface gene and HBV C gene were cloned into two eukaryotic expression vector (pCR3.1 and pcDNA3). The Balb/ c mice were immunized through multiple sites intramuscular injection with pCR3.1-S, pcDNA3-S or pCR3.1-C. Each mouse was boosted 2 weeks after immunization. The anti-HBs and anti-HBc antibodies were detected by ELISA, meanwhile, the stimulatory index of splenocytes on HBsAg or HBcAg was measured by MTT method.RESULTS The liter of special antibody and the stimulatory index of splenocytes from mice coimmunized with pCR3.1-S, pcDNA3-S or pcR3.1-C were significantly higher than that of control. SI of immunized mice injected with pCR3.1-C was higher than that of pCR3.1-S group. CONCLUSION Immunization of C gene and S gene of HBV could produce different cellular immune response of immunized mice, but different vector constructs with the same CMV promotor induced nearly the same immune response in mice.

关 键 词：乙型肝炎 基因免疫 乙型肝炎疫苗 细胞免疫 

分 类 号：R392[医药卫生—免疫学]