扶正排毒片对32例无症状HIV感染者TNF-α及新喋呤的影响  

Effects of Fuzheng Paidu Tablet on Tumor Necrosis Factor-alpha and Neopterin of Thirty-two Asymptomatic HIV Carriers

作　　者：王丹妮[1] 姜枫[1] 郭会军[1] 李华伟[2] 

机构地区：[1]河南中医学院第一附属医院艾滋病临床研究中心,郑州450000 [2]河南中医学院艾滋病研究所,郑州450008

出　　处：《中国中西医结合杂志》2012年第6期744-747,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：科技部国际合作项目资助(No.2007DFB31610);国家973重点课题项目资助项目(No.2006CB504802);国家自然科学基金资助项目(No.30901906;81102575);中国博士后科学基金资助项目(No.20080440743);国家"十一五"科技重大专项资助项目(No.2008ZX10005-002)

摘　　要：目的探讨扶正排毒片对无症状人类免疫缺陷病毒(HIV)感染者血清TNF-α及新喋呤(neop-terin,NPI)的影响。方法应用流式细胞技术检测32例服用扶正排毒片6个月的无症状HIV感染者抗凝全血样本中的CD4+T淋巴细胞,并与治疗前比较;应用ELISA法测定血清TNF-α及NPI水平,并与22名健康体检者比较。结果治疗6个月后无症状HIV感染者CD4+T淋巴细胞由(368.63±111.54)个/mm3上升为(412.72±159.63)个/mm3。无症状HIV感染者治疗前血清TNF-α[(20.05±13.08)nmol/L]、NPI[(9.55±2.52)nmol/L]水平均明显高于健康体检组[分别为(12.20±4.07)nmol/L、(5.91±1.43)nmol/L,P<0.05)],治疗后均比治疗前下降(P<0.05),TNF-α水平[(11.06±4.71)nmol/L]与健康体检组比较差异无统计学意义(P>0.05),NPI水平[(8.08±2.13)nmol/L]仍高于健康体检组(P<0.05)。结论血清TNF-α及NPI水平升高代表的异常免疫激活是无症状HIV感染者的病理因素之一;扶正排毒片可降低无症状HIV感染者血清TNF-α及NPI水平,是其疗效的可能机制之一。Objective To explore the effects of Fuzheng Paidu Tablet （FPT） on serum levels of tumor necrosis factor-alpha （TNF-α） and neopterin （NPI） in patients with asymptomatic human immunodeficiency virus （HIV） carriers. Methods Using flow cytometry detection technology, CD4＋T lymphocyte in anticoagulant blood sample of 32 asymptomatic HIV infection patients who were taking FPT for 6 months was detected and compared with before treatment. The serum levels of TNF-α and NPI were determined using ELISA method and compared with 22 healthy volunteers. Results After 6 months of treatment by FPT, the CD4＋ T lympocyte of asymptomatic HIV carriers increased from （368.63 ± 111.54）/mm3 to （412.72 ±159.63）/mm3. Before treatment the serum levels of TNF-α [ （20.05 ±13.08） nmol/L] and NPI E （9.55 ±2.52） nmol/L] were obviously higher than those of the healthy volunteers [ （12.20±4.07） nmol/L and （5.91±1.43） nmol/L] （P〈0.05）. After 6 months of treatment by FPT, they were lower after treatment than before treatment （ P 〈0.05）. But there was no statistical difference in the serum TNF-α level [ （ 11.06±4.71 ） nmol/L] when compared with the healthy volunteers group （P〉0.05）. But the serum NPI level [（8.08±2. 13） nmol/L] was still higher than that of the healthy volunteers group （P〈0.05）. Conclusions One of the pathological factors for asymptomatic HIV infection is abnormal im- mune activation represented by increased serum levels of TNF-α and NPI. FPT could lower the serum levels of TNF-α and NPI in asymptomatic HIV infection patients, which was one of its possible mechanisms for its efficacy.

关 键 词：无症状HIV感染者 扶正排毒片 肿瘤坏死因子Α 新喋呤 

分 类 号：R512.91[医药卫生—内科学]