持续性心房颤动患者心房阿片肽受体亚型的表达和超微结构变化  

Expression of κ1 opioid receptor and ultrastructure changes in heart atrium from the patients with persistent atria fibrillation

作　　者：张宪伟[1] 蔡小兵[1] 季建华[1] 罗军[1] 衣志勇[1] 张维强[1] 

机构地区：[1]中国人民解放军总参谋部警卫局卫生保健处,北京100017

出　　处：《中华老年医学杂志》2012年第6期458-461,共4页Chinese Journal of Geriatrics

摘　　要：目的观察持续性心房颤动（AF）对心房κ1阿片肽受体mRNA、蛋白表达和超微结构的影响。方法24例窦性心律（SR）组和24例AF组患者，取心房组织，采用反转录聚合酶链反应（RT—PCR）和免疫组化方法，观察其心房κ1阿片肽受体mRNA和蛋白表达的变化。分别采用4例SR组和4例AF组患者的心房组织，用电镜方法分析其线粒体结构和形态变化。结果AF组患者心房κ1阿片肽受体mRNA表达量为262±20，低于SR组196±11，差异有统计学意义（P〈0．05）；AF组κ1阿片肽受体棕色颗粒为（1261±90）个，少于SR组（2325±131）个，差异有统计学意义（P〈0．05）；平均线粒体形态减小，分别为（1．0±0．2）μm与（0．8±0．2）ptm，差异有统计学意义（P〈0．05）。结论AF组κ1阿片肽受体mRNA和蛋白表达下调相关，表明AF时心房组织自我保护能力减弱，并伴有线粒体超微结构重构。Objective To study the effects of persistent atrial fibrillation （AF） on the expression of κ1 opioid receptor and change of ultrastructure in heart atrium. Methods The mRNA and protein expressions of nl receptor in atrial tissue were detected with RT-PCR and immunochemistry, respectively, in 24 patients with AF and 24 patients with sinus rhythm （SR）. Mitochondria were analyzed in the atrial tissue by electron microscopy in 4 patients with AF and 4 cases with SR. Results The κ1 receptor mRNA in patients with AF was lower than in patients with SR （262±20 vs. 196±11, P〈0. 05）, and amount of protein expression was also deduced （1261±90 vs. 2325± 131, P〈0.05）. Mitochondria size was decreased in persistent AF compared with SR[（1.0 ±0.2） /lm vs. （0.8±0.2） μm, P〈0.05]. Conclusions The decrease of κ1 receptor expression suggests reduction of atrial protective capacity in persistent AF, combining with remodeling of ultrastructure.

关 键 词：受体 阿片样 κ 心律失常 心性 心房颤动 基因表达 

分 类 号：R541.75[医药卫生—心血管疾病]