持续小剂量西地那非对良性前列腺增生患者组织内皮素-1及其受体的影响  

The expressions of endothelin-1 and endothelin A/B receptors mRNA in tissue of benign prostatic hyperplasia treated by daily low-dose sildenafil

作　　者：陈洪德[1] 叶雪挺[1] 张奕荣[1] 翁志梁[1] 李澄棣[1] 

机构地区：[1]温州医学院附属第一医院泌尿外科,325000

出　　处：《中华老年医学杂志》2012年第6期500-502,共3页Chinese Journal of Geriatrics

摘　　要：目的观察持续口服小剂量西地那非对良性前列腺增生（BPH）患者前列腺组织中内皮素-1（ET-1）及其受体A、BETAR／ETBR基因表达的影响。方法32例BPH患者随机归入治疗组及对照组，每组各16例。治疗组每日口服西地那非25mg，为期12周。对照组未服用药物。应用免疫组化、酶联免疫吸附法及RT—PCR方法检测并比较两组前列腺组织FT一1及其ETAR的表达。结果ET—1、ETARmRNA及ETBRmRNA在前列腺组织中的表达治疗组明显低于对照组[（53．31±18．56）ng／kg比（83．34±31．38）ng／kg、0．356±0．056比0．624±0．083、0．721±0．083比0．933±0．905，t=-3．295、10．715、6．937，均P〈0．001]。结论持续口服小剂量西地那非能降低BPH组织中ET1及其受体ETA／B基因的表达量。Objective To observe the mRNA expressions of endothelin（ET-1） and endothelin A/B receptors （ETA/B） in tissue of benign prostatic hyperplasia treated by daily low-dose sildenafil. Methods A total of 32 patients with benign prostatic hyperplasia were randomly divided into two groups： treatment（25 mg sildenafi for 12 weeks, n=16） and control （no drug, n=16） groups. Immunohistochemical staining, ELISA and RT-PCR were used to detect the expression levels of ET-1 protein and ET A/B mRNA, respectively. Results The expressions of ET-1 protein and ET A/B mRNA in prostatic tissue were significantly lower in treatment group than in control group[（53.31± 18.56） ng/kgvs. （83.34±31.38） ng/kg, 0. 356 ±0. 056 vs. 0.624±0.083, 0. 721± 0. 083 vs. 0.933±0.905, t=-3.295,10.715, 6. 937, all P〈0. 001]. Conclusions Daily low-dose sildenafil can reduce the expressions of ET-1 and ET A/B receptors mRNA in benign prostatic hyperplasia.

关 键 词：磷酸二酯酶抑制剂 前列腺增生 受体 内皮素A 受体 内皮素B 

分 类 号：R697.3[医药卫生—泌尿科学]