系统性红斑狼疮患者外周血CD4^+调节性T细胞CD25^+和FOXP3^+的表达及意义  

Expression and significance of CD4^+CD25^+FOXP3^+ regulatory T cells in patients with systemic lupus erythematosus

作　　者：郭露露 张缪佳 季晓辉 谈文峰 吕凌 王艳艳 王嫱 吕成银 

机构地区：[1]南京医科大学第一附属医院风湿免疫科,江苏省210029

出　　处：《江苏医药》2012年第11期1307-1310,共4页Jiangsu Medical Journal

基　　金：国家"十一五"科技支撑计划(2008BAI59B02)

摘　　要：目的探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中CD4+T细胞CD25和FOXP3的表达及其临床意义。方法流式细胞术检测20例SLE患者(9例为狼疮性肾炎)及10例健康对照组PBMC中CD4+T细胞中CD25及Foxp3的表达。结果 SLE患者CD4+CD25highT细胞中FOXP3+T细胞的表达明显低于健康对照组(P<0.05),与SLE疾病活动指数SLEDAI积分明显负相关(r=-0.514,P<0.01),与补体C4正相关(r=0.362,P<0.05),与ESR及CRP负相关(r分别=-0.23、-0.216,P<0.05),与外周血免疫球蛋白IgG、IgA、IgM及补体C3水平无明显相关性。将SLE患者分为狼疮性肾炎(LN)组及非肾损(SLE-NR)组,两组CD4+CD25highT细胞及CD4+CD25lowT细胞中FOXP3+T细胞的表达均明显低于健康对照组(P<0.05);LN组CD4+T细胞中CD25high及CD25low的表达均较SLE-NR组增高(P<0.05)。结论 SLE患者外周血中CD4+CD25high T细胞中FOXP3+的表达水平明显降低,且与疾病活动性相关,而LN患者代偿增高的无FOXP3表达的CD4+CD25+T细胞可能与其发病机制有关。Objective To investigate the expression of and significance of CIM＋ CD25＋ FOXP3＋ regulatory T cells in patients with systemic lupus erythematosus （SLE）. Methods The expressions of CD25 and FOXP3 in CD4＋ T cells of peripheral blood were detected using flow cytometry in 20 SLE patients including nine lupusnephritis（group SLE） and 10 healthy volunteers （group C）. Results The expression of FOXP3 in CD4＋ CD25highT cells was lower in group SLE than that in group C（P〈0. 05）,which was negtively correlated with SLEDAI （r=-O. 514,P〈0. 01） and the levels of ESR and CRP（r=-0. 23 and r=-0. 216, P〈0. 05）, positively correlated with the levels of C4 （r=0. 362, P〈0. 05）, hut not with the levels of C3, IgG, IgA and IgM. The expressions of FOXP3 in CD4＋CD25highT cells and CD4＋ CD25lowT cells were lower in group SLE than those in group C （P〈0. 05）. The expressions of CD25high and CD25lowin CD4＋ T cells were higher in SLE patients with lupusnephritis than those without（P〈0. 05）. Conclusion The expression of FOXP3 in CD4＋ CD25high T cells is decreased in SLE patients and correlated with disease activity. The increased CD4＋ CD25＋ FOXP3- T ceils without FOXP3 expression may play an important role in the development of lupusnephritis in SLE patients.

关 键 词：调节性T细胞 系统性红斑狼疮 狼疮性肾炎 

分 类 号：R593[医药卫生—内科学]