大鼠断尾再植后超短波治疗影响鼠尾成活的相关性研究  

A correlative study of ultrashortwave therapy affecting survival of rat tail＇replantation

作　　者：谭龙[1] 高文山[2] 席阿丽[3] 王聪[2] 陈首英[4] 赵燕燕[5] 邸科前[5] 杨信才[1] 瓮生彬[6] 

机构地区：[1]河北大学附属医院康复科,河北省保定市071000 [2]河北大学附属医院骨科,河北省保定市071000 [3]兰州军区边防军医训练大队药化教研室 [4]河北大学附属医院公共卫生学院流行病学教研室,河北省保定市071000 [5]河北大学附属医院医学实验中心,河北省保定市071000 [6]河北大学附属医院急诊科,河北省保定市071000

出　　处：《中华显微外科杂志》2012年第3期210-214,共5页Chinese Journal of Microsurgery

基　　金：河北省2009年医学科学研究重点课题计划指导性课题（20090573）

摘　　要：目的探讨超短波对鼠断尾再植后血管危象的影响及作用机制。方法制备断尾再植模型：除尾侧静脉、尾骨保留，断离尾，吻合尾动脉。80只3月龄健康sD大鼠随机分4组：空白组（0组）、模型组（1组）、对照组（2组）和超短波（USW）组（3组）。3组又分高剂量组（3A组）、低剂量组（3B组）。0组结扎尾动脉，其余组均吻合。1组正常管理；2组术后5d天每天1次予罂粟碱液；3组术后5d每日1次予局部吻合口处USW治疗20min：3A组USW剂量为3档，50mA，3B组剂量为2档，28mA。观察鼠尾成活情况10d。分组前测鼠尾吻合口近、远侧皮温差1次，每日测1次。分组前和术后第8h，测血浆NO。结果鼠尾成活率：断尾组0％，断尾再植组44％，P=0．002；断尾再植的各组（1、2、3A、3B组）间成活率比较：3B组〈2组〈1组〈3A组，P=0．022；3B组与1组比较，P=0．031；2组与1组比较，P=0．221。皮温差变化量术后较术前比较：术后第1天：3A组〈1组，P=0．028；术后第6天：3A组〈3B组〈1组〈2组，P=0．024；术后第7天：3B组〈1组〈3A组〈2组，P=0．001。NO术后较术前比较：3B组〉3A组〉2组〉1组〉0组，P=0．000。结论实验大鼠断尾再植模型可行：USW治疗能提高鼠断尾再植存活率，在术后第1、6、7天，能减小皮温差，提高术后第8hNO浓度，防治血管危象。Objective To research the effect mechanism of ultrashortwave in the correlation of ultrashortwave and the tail replantation, provide the experiment basis of clinical practice of prevention and cure for the vascular crisis after micromodule anastomosis. Methods Eighty Sprague-Dawley （SD） rats of clean grade were 3-month-old, female, and were divided into four groups： control group （group 0）, model group （group 1）, contrast group （group 2）, ultrashortwave （USW） group （group 3）. The preparation of tail replantation model was cut off soft tissue except for caudal veins on both sides of the tail. The coccyx was not broken away from tail. At last, the audal artery under abdominal main centre ditch was anastomosed. In ex- periment process, the USW group was divided into high dosage group （group 3A） and low dosage group （group 3B）. The caudal arterys were ligated and not anastomosed in the group 0. Caudal arterys in other groups were anastomosed. Rats in the group 0 and group 1 received no treatment, normal management after the operation. Rats in the group 2 were given abdominal cavity injection of papaverin liquid immediately, then once a day to 5 clays after the tail replantation.Rats in the group 3 were immediately given USW therapy of twenty minutes on the anastomosis section, and then once a day for 5 clays after the tail rep]antation. The USW dosage of group 3A was 3th grade and 50mA. The USW dosage of group 3B was 2th grade and 28mA. The survival rate of the rat tails was observed after the tail replantation for 10 day. Before being grouped, it wasmeasured that the tail skin temperature diference between near and far side of anastomosis section. After the tail relpantation, the temperature diference was inspected daily for 10 postoperative days hence. Before rats were grouped and the eighth hour after the tail replantation, rats were collected blood plasma specimens and mea- sured contents of nitric oxide with destination colorimetric mathods of nitric oxide. Results Carrying out

关 键 词：断尾再植 超短波 血管危象 血管内皮细胞 一氧化氮 皮温差 

分 类 号：R654.3[医药卫生—外科学]