低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响  

Effect of oligomeric grape seed proanthocyanidins on prevention isoproterenol induced cardiomyocytes hypertrophy

作　　者：高杉[1] 黄春霞[2] 黄玲玲[1] 俞婷婷[1] 汪星辉[1] 张野[2] 

机构地区：[1]安徽医科大学基础医学院药理学教研室,安徽合肥230032 [2]安徽医科大学第二附属医院麻醉科,安徽合肥230061

出　　处：《中国临床药理学与治疗学》2012年第5期497-501,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：教育部高等学校博士学科点基金(20103420120002);国家自然科学基金(81073088);安徽医科大学博士启动基金(XJ200709)

摘　　要：目的:在原代培养的新生大鼠心肌细胞上观察低聚葡萄籽原花青素(oligomeric grape seed proanthocyanidins,GSP)对异丙肾上腺素(isoproterenol,Iso)诱导心肌细胞肥大的影响。方法:采用SD大鼠乳鼠原代心肌细胞培养法,BCA法测定心肌细胞总蛋白含量;流式细胞仪检测心肌细胞内氧自由基(reactive oxygen species,ROS)含量;比色法检测丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果:1×10-5 mol/L Iso能明显诱导心肌细胞肥大,GSP可剂量依赖性地抑制Iso诱导心肌细胞肥大,抑制细胞内ROS的生成,降低MDA含量、提高SOD活性。结论:GSP对Iso诱导的心肌细胞肥大具有明显的逆转作用,其机制至少部分与抑制ROS生成、降低氧化应激水平有关。To investigate the effect of oligomeric grape seed proanthocyanidins （GSP） on neonatal cardiomyocytes hypertrophy induced by isoproterenol （Iso）. METHODS： Hypertro- phy in cultured neonatal rat cardiac myocytes was induced by Iso. Total protein content was measured by BCA method; reactive oxygen spe-cies （ROS） were detected by flow cytornetry; colorimetric method was employed to measured MDA content and SOD antivity. RESULTS： 1 ×10-s mol/L Iso can activate β-AR completely and induce cardiomyocytes hypertrophy markedly. GSP therapy potently inhibited the increase ofprotein content, ROS generation and MDA con- tent, inhibited the decrease of SOD activity with a dose-dependent manner. CONCLUSION.. GSP possess protective effect against Iso induced car- diomyocytes hypertrophy, this may be related to reducing the ROS generation and oxidative stress level, at least in part.

关 键 词：低聚葡萄籽原花青素 异丙肾上腺素 心肌细胞肥大 氧自由基 丙二醛 超氧化物歧化酶 

分 类 号：R965.2[医药卫生—药理学]