异丙酚预处理对缺氧/复氧后培养海马神经元金属硫蛋白-3蛋白表达的影响  

The impact of propofol pretreatment on hypoxia/reoxygenation in cultured hippocampal neurons metallothionein-3 protein expression

作　　者：何建国[1] 黄长顺[1] 姜娟[1] 胡烨[1] 曹刚[1] 刘振伟[1] 

机构地区：[1]宁波市第一医院麻醉科,浙江宁波315010

出　　处：《中国临床药理学与治疗学》2012年第5期517-520,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:本研究观察异丙酚对培养海马神经元缺氧复氧后损伤反应的影响及金属硫蛋白-3(metallothionein-3,MT-3)在其中的作用。方法:培养7～10d海马神经元,以不同浓度的异丙酚(50,150,250μmol/L)预处理后24h后,制备缺氧4h后复氧24h模型(H/R),Western blot法检测MT-3蛋白表达水平;以MT-3特异性siR-NA沉默MT-3表达后,采用MTT法观察异丙酚对H/R海马神经元存活率的影响。结果:在H/R条件下,异丙酚可浓度依赖性促进MT-3的蛋白表达。以MT-3siRNA抑制MT-3蛋白表达后,异丙酚提高神经元存活率的作用消失。结论:异丙酚具有神经元保护效应,这一作用可能与其上调MT-3的蛋白表达有关。ABSTRACT AIM： To observe the effects of propofol on caltured hippocampal neurons hy- poxia and reoxygenation damage response and metallothionein protein-3 （metllothionein-3, MT- 3） in which the role. METHODS： Hippoeampal neuron cells were cultured for 7- 10 days for use. Hypoxic conditions were attained 4 h and reoxygenation was achieved with normoxia con-ditions for another 24 h. The expression revel oi metallothionein-3 （MT-3） was detected by Western blot; By using MT-3 specific siRNA, MT-3 was silenced and the effect of propofol on cell survival rate was evaluated by MTT method RESULTS： In cultured hippocampal neuroncells, pretreatment with different concentrations of propofol （50, 150, 250 mol/L） for 24 h en- hanced MT-3 expression level after hypoxia/ reoxygenation. When MT-3 expression was si- lenced by specific siRNA, propofol failed to im- prove hippocampal neuron cell survival rate. CONCLUSION. MT-3 exerts hippocampal neu- ron protective effect, which may possibly be me- diated by the upreguation of MT-3 expression.

关 键 词：异丙酚 金属硫蛋白-3 海马神经元 缺氧/复氧 

分 类 号：R965.2[医药卫生—药理学]