长期中等强度运动对小鼠骨骼肌HIF-1α mRNA的表达及葡萄糖转运的影响  

Effects of long-term exercise with moderate intensity on the expressions of hypoxia inducible factor-1α mRNA and glucose transporters in skeletal muscles of mice

作　　者：谢康玲[1] 刘遂心[1] 蔡颖[1] 张文亮[1] 

机构地区：[1]中南大学湘雅医院康复医学科,长沙410008

出　　处：《中国康复医学杂志》2012年第6期514-518,共5页Chinese Journal of Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(30672213)

摘　　要：目的:观察长期中等强度运动对小鼠骨骼肌血管内皮生长因子-A(VEGF-A)、葡萄糖转运体1(GLUT-1)、葡萄糖转运体4(GLUT-4)mRNA表达的影响,在此基础上进一步观察运动对缺氧诱导因子1α(HIF-1α)mRNA表达的影响,初步探讨运动对小鼠骨骼肌葡萄糖转运的影响及其可能机制。方法:健康雄性昆明小鼠24只随机分为运动组和对照组,运动组小鼠给予12周中等强度跑步训练。末次运动后处死所有小鼠,取双侧腓肠肌,光镜及电镜观察显微及超微结构;实时PCR法测定小鼠骨骼肌VEGF-A、GLUT-1、GLUT-4及HIF-1αmRNA的表达。结果:①运动组小鼠较对照组体重明显增加(P<0.05);②骨骼肌肌细胞数目增加、肌纤维增粗,肌原纤维的直径增加,线粒体数目增加;③运动组小鼠骨骼肌VEGF-A、GLUT-1、GLUT-4及HIF-1αmRNA表达明显高于对照组(分别增加1.68、1.14、2.31、1.92倍,P分别<0.05、0.01、0.01、0.01)。结论:长期中等强度运动可导致小鼠体重增加、肌纤维增粗、线粒体数目增加,VEGF-A mRNA表达增加;可增加骨骼肌GLUT-1、GLUT-4 mRNA的表达,其机制可能与上调骨骼肌HIF-1αmRNA表达有关。Objective： To investigate the effect of long-term exercise with moderate intensity on the expressions of glucose transporter-1 （GLUT-1） and glucose transporter-4 （GLUT-4） mRNA, as well as of hypoxia inducible factor-1α （HIF-1α） and vascular endothelial growth faetor-A （VEGF-A） mRNA in skeletal muscles of mice and explore its possible mechanisms. Method： Kunming male mice （n=24） were randomly divided into exercise group and control group. The mice in exercise group were forced to run with moderate intensity on a treadmill. After 12 weeks of exercise, all mice were sacrificed immediately and both sides of gastrocnemius were harvested. The middle part of tissue samples were prepared for H ＆ E staining, and analysed under light microscope and transmission electron microscope. In addition, part of tissues were used to check gene expression by real-time PCR. Result： （1）Compared to control group, the body weights of exercise group increased significantly（P〈0.05）. （2）The number of muscle cells increased, the size of muscle fibers thickened, the diameter of myofibril and the amount of mitochondria increased significantly. （3）The expressions of VEGF-A mRNA, GLUT-1 mRNA, GLUT-4 mRNA,and HIF-1α mRNA in exercise group were significantly higher （increased by 1.68, 1.14, 2.31 and 1.92-fold sep- arately, P 〈0.05, 0.01, 0.01, 0.01 separately） than those of control group. Conclusion： （1）Long-term exercise with moderate intensity can cause weight gain, thickening of skeletal muscle fi- bers, increasing of mitochondria amount and expression of VEGF-A mRNA; （2）Long-term exercise with moderate intensity can increase the expressions of GLUT-1 mRNA, GLUT-4 mRNA in skeletal muscles. Induction of HIF-1α mRNA expression in skeletal muscles by exercise might be one of the possible mechanisms.

关 键 词：运动 缺氧诱导因子1Α 骨骼肌 葡萄糖转运体 小鼠 

分 类 号：G804.2[文化科学—运动人体科学]