不同烤烟品种衰老叶片的无机氮积累及其生理调控  

Inorganic N Accumulation and Its Physiological Regulation in Senescing Leaves of Flue-Cured Tobacco (Nicotiana tabacum L.) Cultivars

作　　者：段旺军[1,2] 李东亮 戴亚 杨铁钊[2] 李宁 汪长国 

机构地区：[1]川渝中烟工业有限责任公司技术研发中心,成都610066 [2]河南农业大学烟草学院,郑州450002

出　　处：《植物生理学报》2012年第6期544-548,共5页Plant Physiology Journal

基　　金：川渝中烟工业有限责任公司博士后项目(川渝烟工技研[2010]347号-20)

摘　　要：以不同烤烟品种‘红花大金元’和‘中烟100’为试验对象,研究同一生育期不同部位叶片的无机氮积累及其与氮素代谢和氨挥发的关系。结果表明,烟草植株下部衰老叶片(第5片叶)NO3-和NH4+的含量要高于中部叶(第10和第15片叶)和上部叶(第20片叶),并且‘红花大金元’下部叶NO3-和NH4+的含量比‘中烟100’显著偏高;‘中烟100’植株各部位叶片的氨气补偿点比‘红花大金元’高。两个品种在第5到第20叶位间的谷氨酰胺合成酶(GS)、硝酸还原酶(NR)和谷氨酸脱氢酶(GDH)活性大小及其变化不一致,是叶片无机氮积累存在品种间差异的生理基础。Accumulation of inorganic nitrogen and its relationship with nitrogen metabolism and ammonia volatilization of different positions of leaves were investigated with different flue-cured tobacco cultivars as experimental subjects. The results indicated that the contents of NO3^- and NH4^＋ in bottom senescent leaves （leaf 5） were higher than those in middle （leaf 10 and 15） and top leaves （leaf 20） of tobacco, and the contents of NO3^- and NH4^＋ of ＇Honghuadajinyuan＇ were significantly higher than those of ＇Zhongyan100＇. And ＇Zhongyan 100＇ exhibited higher apoplastic NH3 compensation points than ＇Honghuadajinyuan＇. The glutamine synthetase （GS）, nitrate reductase （NR） and glutamate dehydrogenase （GDH） activities and their changes in leaves of two cultivars were different. These results were physiological basis of the accumulation of inorganic nitrogen differences between cultivars.

关 键 词：烟草 无机氮 氨气补偿点 氮素代谢 

分 类 号：S572[农业科学—烟草工业]