携带肠道病毒71型中和线性表位重组流感病毒的构建  

~ Construction of recombined influenza viruses with a neutralizing linear epitope of enterovirus 71

作　　者：吴海波[1] 沃恩康[1] 尤金彪[1] 王怡婷[1] 王巧刚[1] 郭潮潭[1] 

机构地区：[1]浙江省医学科学院生物工程研究所,杭州310013

出　　处：《国际流行病学传染病学杂志》2012年第3期157-160,共4页International Journal of Epidemiology and Infectious Disease

基　　金：浙江省重大科技专项（2008C03003.1）;浙江省科技计划项目（2010C33166）;浙江省医药卫生科研项目（2008ZX001,2009A005）

摘　　要：目的获得能够携带肠道病毒71型（EV71）中和线性表位的重组流感病毒。方法通过PCR方法，在流感病毒HA基因中插入EV71中和线性表位序列，获得重组质粒；进行反向遗传学操作，构建重组流感病毒。结果获得了2个重组质粒pPolⅠ／Ⅱ／WSN-HA-SP55和pPolⅠ／Ⅱ／WSN-HA-SP70，并构建了能够分别携带EV71中和线性表位SP55和SP70的2株重组流感病毒，重组病毒3次传代血凝效价都保持在2^7和2^5HAU，具有较好的稳定性。结论通过反向遗传学操作，构建出携带Ev71中和线性表位的重组流感病毒，为研制预防手足口病重组流感病毒载体疫苗奠定基础。Objective To construct recombined influenza viruses with a neutralizing linear epitope of enterovirus 71 （EV71 ）. Methods For getting recombined plasmids, the sequence of a neutralizing epitope of EV71was inserted into influenza virus hemagglutinin（HA） gene by PCR, and constructed recombined influenza viruses by reverse genetics. Results In this study, two recombined plasmids pPol I/lI/WSN-HA-SP55 and pPol I/II/WSN-HA-SP70 were constructed, and two recombined influenza viruses with a neutralizing linear epitopes SP55 and SP70 were obtained, respectively. In three passages, they had a good stability with 27 and 25 HAU. Conclusions By reverse genetics, two recombined influenza viruses have been constructed , and the two viruses will have a better developing foreground for using as candidate strains of live vaccine for preventing hand-foot-and-mouth disease.

关 键 词：肠道病毒属 流感病毒载体 中和线性表位 疫苗 

分 类 号：R3[医药卫生—基础医学]