尿液分离肠杆菌科细菌整合子分子特征及其与耐药性的关系  

Molecular characteristics of integrons among Enterobacteriaceae isolated from urine and their relations withantibiotic resistance

作　　者：王卫忠[1] 俞燕[1] 戴妹贤[2] 孙彬[3] 

机构地区：[1]浙江省人民医．院检验中心,杭州310014 [2]温州医学院,325035 [3]浙江中医药大学,杭州310053

出　　处：《国际流行病学传染病学杂志》2012年第3期161-164,共4页International Journal of Epidemiology and Infectious Disease

基　　金：浙江省医学重点学科基金（07-010）

摘　　要：目的了解尿液分离肠杆菌科细菌中整合子及相关基因盒的分布，分析整合子与细菌耐药性的关系。方法选取111株临床上非重复分离自尿液样本中的肠杆菌科细菌菌株，经全自动细菌分析系统鉴定并检测其对临床常用抗生素的耐药性，PCR检测第1，2，3类整合酶基因（intl1、intl2、intl3），对于第1类整合酶阳性菌株用PCR扩增可变区基因盒。结果111株临床非重复分离自尿液样本的肠杆菌中有69株（62％）检测到第1类整合酶基因，12株（11％）检测到第2类整合酶基因，未检出第3类整合酶基因。共检测出6种不同长度的可变区片段：800bp片段不含基因盒，1500bp片段为aadB，1800bp片段为atdA2，2200bp片段为aac（6＇）-Ib-cm／A1，3500bp片段为aadB-catB3-aadA1，3000bp为dfrA32。dfr32是首次在1株奇异变形杆菌中检测出的一种新的甲氧苄啶耐药基因。第1类整合子阳性菌株对多种抗菌药物的耐药率均高于第1类整合子阴性菌株。结论第1类整合子及相关基因盒在肠杆菌科细菌中分布广泛，与细菌耐药性关系密切。Objective To investigate the distribution of integrons and integrated gene cassettes in Enterobacteriaceae isolated from urine and analyze their relations between integrons and drug resistance. Methods One hundred and eleven clinical non-repeated isolates of Enterobacteriaceae from urine samples were selected. Automatic bac- terial analysis system was used to identify and test antimicrobial susceptibility of 111 clinical isolates. Class 1, 2 and 3 in- tegron integrase genes（ intll, intl2, intI3） were screened by PCR, the inserted gene cassettes of variable regions in intll positive isolates were detected by using PCR combined with sequence analysis. Results Among 111 Enterobacteriaceae clinical non-duplicate isolates from urine, intll were detected in 69 isolates（62 % ） and intl2 in 12 isolates（ 11% ）, no intI3 gene was detected. Six kinds of variable fragments of different lengths were detected in intll positive isolates：800 bp fragment did not contain gene cassette, 1500 bp fragment containd gene cassette aadB, 1800 bp fragment containd gene cassette aadA2, 2200 bp fra^nem was gene cassette array aac（6＇ ） -Ib-cmIA1, 3500 bp fragment was aadB-catB3- aadA1 and 3000 bp was dfrA32, dfrA32 was detected for the first time in a Proteus mirabilis as a new kind of trimetho- prim resistance gene. The resistance rates of many antimicrobial agents of class 1 integron-pesitive strains tested in this study were higher than those of the negatives. Conclusions Class 1 integrons as well as their associated gene cassettes are widely distributed among Enterobacteriaceae and closelv related to antimicrobial agent resistance.

关 键 词：整合子类 基因盒 耐药性 

分 类 号：R446.5[医药卫生—诊断学]