苗期干旱对花生抗病相关基因PnAG_3的表达及耐旱生理指标的影响  

Effects of Drought during Peanut(Arachis hypogaea L.) Seeding Stage on Disease Resistance Gene PnAG_3 Expression and Physiological Index Related to Drought Resistance

作　　者：刘宇[1,2] 李春娟[1] 郑奕雄[3] 闫彩霞[1] 张廷婷[1] 单世华[1] 

机构地区：[1]山东省花生研究所,青岛266100 [2]中国海洋大学,青岛266100 [3]仲恺农业工程学院,广州510225

出　　处：《农业生物技术学报》2012年第6期642-648,共7页Journal of Agricultural Biotechnology

基　　金：现代农业产业技术体系建设专项资金(No.CARS-14);山东省现代花生产业技术体系;山东省良种工程"花生高产;优质;多抗基因资源发掘与种质创新利用研究"项目

摘　　要：为确定抗病基因PnAG3与花生抗旱性关系,本实验利用土壤盆栽试验,通过控水模拟干旱条件,利用荧光定量方法分析不同程度干旱、苗期不同抗病花生(Arachis hypogaea L.)品种PnAG3基因的表达变化,同时测定它们耐旱生理指标的变化。结果表明,抗病品种在中度干旱胁迫下超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(catalase,CAT)活力分别升高了3.15、2.55和4.95倍,而不抗病品种在3种处理下差异不显著;丙二醛(malondialdehyde,MDA)含量在不同干旱程度下均有所升高,且不抗病品种升高幅度大于抗病品种;基因PnAG3的表达量在抗病品种中要高于不抗病品种,但抗病品种在中度和重度干旱胁迫下基因表达量出现的峰值较不抗病品种早,与CAT活性的变化相似。整体来看,SOD呈持续上升趋势,而基因PnAG3表达量、POD、CAT和MDA呈现先上升后下降的趋势,结合耐旱生理指标及抗病相关基因PnAG3的变化情况,基因PnAG3表达量变化与耐旱生理指标的变化有相关性,且在抗病品种中基因PnAG3的表达量大于不抗病品种,推测基因PnAG3的功能与花生抗逆性有关。本研究为进一步研究花生抗性育种和种质筛选提供依据。With the changes of soil moisture, the PnA G3 expression changed in the several peanuts （anti-Aspergillus flavus species J11, HY22 and susceptible peanut species JH1012） growing in the pot, while their physiological index related to drought resistance was also changed. The results showed that the Superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT） activities of anti-Aspergillus flatus species had increased 3.15, 2.55 and 4.95 times in 40%-55% of field maximum water capacity treatment group, respectively. But the SOD, POD and CAT activity of susceptible species in the three treatment groups had no significant difference. Malondialdehyde（MDA） contents were increased in all treat groups, and it washigher in susceptible peanut species than that of anti-Aspergillus flavus species. PnA G3 expression levels were higher in anti-Aspergillus flavus species than that of susceptible peanut species. But the maximum expression level of anti-A spergil!us flavus species was appeared earlier than that of susceptible species which was similar to the CAT activity changes. Overall, SOD was continued to rise. But the PnAG3 gene expression, and POD, CAT and MDA showed a tendency of ＂up-down＂. Combined with the physiological index related to drought resistance and the PnA G3 expression changes, there was a correlation between the variation trend of PnA G3 expression and adversity physiological indexes. And the PnA G expression in resistant varieties was higher than that of susceptible varieties. So that, the function of PnA G3 may be relevant to the peanut stress resistance.This study provides a basis for further research on resistance breeding and germplasm screening of peanut.

关 键 词：花生 干旱胁迫 荧光定量 逆境生理 NBS—LRR 

分 类 号：S512.1[农业科学—作物学]