基于高密度SNP芯片技术对中国西门塔尔牛SCD1基因与肉质性状的相关性分析  

Association of SCD1 Gene with Meat Quality Traits in Chinese Simmental Based on High-density SNP Chip

作　　者：张立敏[1] 张猛[1] 周正奎[1] 刘利[1] 刘喜冬[2] 陈翠[1] 陈晓杰[1] 朱淼[1] 袁峥嵘[1] 李姣[1] 许尚忠[1] 高会江[1] 高雪[1] 张路培[1] 李俊雅[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193 [2]东北农业大学动物科技学院,哈尔滨150030

出　　处：《畜牧兽医学报》2012年第6期878-886,共9页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业部专项(Nycytx-38);国家自然基金(30871774);转基因生物新品种培育重大专项(2009ZX08007-005B);“十一五”转基因重大专项(2008ZX08007-2);优质肉牛新品种(系)选育与关键技术研究及示范(2011BAD28B04);基于高密度SNP建立中国肉牛群体全基因组选择优化方案(2010jc-2)

摘　　要：试验借助高密度SNP芯片技术在整个基因组范围内对223头中国西门塔尔牛群体进行基因分型,在SCD1基因上发现7个SNPs位点,对各SNP位点与西门塔尔牛肉质性状进行相关性分析。结果表明,SCD1基因的7个SNPs位点均与大理石花纹等级显著相关(P<0.05或P<0.01);A10050C、A13655G、C14790T和A15565G4个位点对剪切力影响显著(P<0.05);位点A13655G对肌内脂肪含量影响显著(P<0.05);位点A10050C、C14790T、A15565G不同基因型个体间脂肪颜色差异显著(P<0.05)。单体型分析结果显示,7个SNPs位点共构成6种单体型和14种单体型组合。单体型组合H3H6与高肌内脂肪含量极显著相关(P<0.01);单体型组合H6H6与优质大理石花纹极显著相关(P<0.01);单体型组合H4H6与高剪切力值显著相关(P<0.05);各单体型组合对肉色和脂肪颜色影响不显著。SNPs of in a population wit the whole genome were genotyped through high-density SNP chip technology h 223 Chinese Simmental. Seven SNPs were found in SCD1 gene. The results of association of the seven loci with meat quality traits showed that the loci were all significantly correlated with marbling score （P〈0. 05 or P〈0. 01）. The effect of different genotypes at A10050C, A13655G, C14790T and A15565G on sheare force were significant（P〈0.05）. The locus A13655G was correlated with intramuscular fat（IMF） content, and individuals with AAgenotype has higher IMF （P〈0. 05）. The effect of different genotypes at locus A10050C, C14790T and A15565G on fat color were significantly different（P〈0.05）. The result of haplo- type analysis showed that 6 haplotypes and 14 haplotype combinations were detected. For IMF content, the individuals with haplotype combination H3H6 was significant higher than others at 0.01 level while the individuals with haplotype combination H6H6 had a higher marbling score and the individuals with haplotype combination H4H6 had a higher sheare force. The effect of the haplotype combinations on meat color and fat color was not significant.

关 键 词：高密度SNP芯片 中国西门塔尔牛 SCD1基因 肉质性状 单体型 

分 类 号：S823[农业科学—畜牧学] Q347[农业科学—畜牧兽医]