Clade2．3．2H5N1亚型禽流感疫苗候选株的构建  

Generation of Clade 2.3.2 H5N1 Subtype Avian Influenza Vaccine Candidate

作　　者：张妍[1] 张文俊[1] 李群辉[1] 刘晓文[1] 刘文博[1] 刘秀梵[1] 

机构地区：[1]扬州大学兽医学院农业部畜禽传染病学重点开放实验室,扬州225009

出　　处：《畜牧兽医学报》2012年第6期915-921,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：现代农业产业技术体系建设专项资金(nycytx-41-G07);国家重点基础研究发展计划(973计划)(2011CB505003)

摘　　要：近年来Clade2.3.2H5N1亚型的禽流感病毒逐渐成为我国及越南等其他一些国家流行的优势毒株,并呈现出一定变化规律的氨基酸进化趋势。本研究以A/Puerto Rico/8/34(H1N1)(PR8)AIV为内部基因供体,以Clade2.3.2H5N1亚型AIV A/chicken/Yangzhou/1117/2011(YZC3)为表面抗原血凝素(HA)和神经氨酸酶(NA)基因供体,通过反向遗传操作,在符合人类疫苗生产标准的COS-1细胞中救获低致病性的疫苗毒株。结果成功拯救出1株重组病毒rYZC3,该病毒在鸡胚和MDCK细胞上均具有较好的繁殖能力,对SPF鸡和鸡胚无致病性。本研究为防控当代流行的H5亚型禽流感提供了良好的疫苗候选株。Currently, H5N1 viruses in Clade 2.3.2 are predominant viruses which prevail in China and other countries, and occurrence of a certain evolutionary trends of amino acid changes. In this report, a reassortant virus containing the HA and NA genes from an new isolate A/chicken / Yangzhou/lll7/20ll（YZC3） and the backbone A/Puerto Rico/8/34 （H1N1） （PR8） strain were constructed. The virus was rescued from the COS-1 cell line and prepared as inactivated whole-vi- rus vaccine. Vaccine virus was avirulent to SPF chickens and embryonated chicken eggs, replica- ting well in eggs and MDCK cells. The results indicate that the reassortant viruses is suitable for vaccine manufacturing and being used as reference vaccine virus against the H5N1 Clade 2.3.2 vi- ruses.

关 键 词：H5亚型 Clade2.3.2 禽流感病毒 反向遗传 

分 类 号：S852.659.5[农业科学—基础兽医学]