猪捷申病毒HB株的分离与全基因序列分析  

Isolation and Sequence Analysis of Complete Genome of Porcine Teschovirus HB Strain

作　　者：张晓杰[1,2] 刘业兵[1] 汤德元[2] 朱向博[1] 

机构地区：[1]中国兽医药品监察所,北京100081 [2]贵州大学动物科学学院,贵阳550025

出　　处：《畜牧兽医学报》2012年第6期937-942,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(31172341;31172349);十一五国家科技支撑计划项目(2007BAD86B04)

摘　　要：为分析河北省某规模化猪场猪捷申病毒(PTV)的遗传变异和进化关系,作者成功分离到PTV HB株,对其进行了毒力测定和动物回归试验,设计引物进行了全基因序列测定,并与国内外42株PTV全基因序列进行了同源性比较。结果表明猪捷申病毒HB株病毒滴度为10-6.4 TCID50.mL-1,健康仔猪接毒28d后均出现明显猪捷申病症状,该毒株全基因组序列长度为7 090bp。系统进化树分析表明,猪捷申病毒HB株(JQ664746)属于PTV-8型血清型,和国内分离的PTV-jilin/2003株(GQ293092)同源性最高,从而为进一步研究该毒株的遗传变异提供参考。This experiment was conducted to study the genetic variation and phylogenetic analysis of Porcine teschovirus （PTV） HB strain isolated from a pig farm in Hebei Province. ST cells and animal infection experiments were conducted after the virus was originated from clinical tissue samples successfully, and the genome sequence of PTV HB strain had been determined, compara- tive genome analysis was performed with other 42 PTV strains in GenBank. The virus titer was 10-64TCID50 · mL 1 , and the infected pigs showed the same clinical signs and homological chan- ges as natural infected ones after 28 days, and the complete genome sequence was composed of 7 090 nucleotides. Phylogenetic tree analysis demonstrated that PTV HB strain （JQ664746） be- longed to serotype 8 and had a very closely genetic relationship with PTV-jilin/2003 strain （GQ293092） isolated from Jilin Province, China. Our results provided an essential reference for investigating the genetic variation of PTV HB strain.

关 键 词：猪捷申病毒HB株 病毒分离 全基因序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]