基于线粒体12S基因对青海地区细粒棘球蚴种群遗传多态性研究  

Genetic Polymorphisms of Echinococcus granulosus in Qinghai Based on the Mitochondrial DNA 12S Gene

作　　者：延宁[1] 聂华明[1] 蒋忠荣[2] 邓世金 余华[3] 严玉宝[3] 达瓦次仁 谢跃[1] 付彦[1] 杨德英[1] 郝桂英[1] 古小彬[1] 杨光友[1] 

机构地区：[1]四川农业大学动物医学院,雅安625014 [2]四川省甘孜州畜牧科学研究所,康定626000 [3]四川出入境检验检疫局,成都610041 [4]西藏自治区动物疫病预防控制中心,拉萨850000

出　　处：《畜牧兽医学报》2012年第6期972-978,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支持计划项目(2006BAI06B09);国家质检总局科技计划项目(2009IK019)

摘　　要：本研究旨在分析青海地区细粒棘球蚴的种群基因多态性,为细粒棘球蚴病的防控提供基础资料。对采自青海地区的42株细粒棘球蚴(33株采自绵羊肝脏,9株采自绵羊肺脏)进行了线粒体12S基因的全序列测序并构建了NJ系统发生树。结果显示:在本研究样品的线粒体12S基因序列中共检测出5种单倍型(即H1～H5),其中以单倍型H5为主(占32株),并且系统发生树分析支持这一结果。单倍型多样性(H)和核苷酸多样性(Pi)分别为0.418、0.000 66,与E.granulosus G1(AF297617)的12S基因序列的核苷酸相似性达到99.86%以上。采自青海地区的42株细粒棘球蚴均鉴定为E.granulosus sensu stricto(基因型G1-G3),在检测出的5种单倍型中,单倍型H1～H4是本地区特有的单倍型。The aim of the current study was to investigate the genetic polymorphisms of Echino- coccus granulosus obtained from sheep in Qinghai Province, and provide the foundational data for prevention and control of echinococcus disease. The complete mitochondrial DNA 12S genes of 42 isolates（33 were from liver and 9 were from lung）were sequenced, and phylogenetic analyses were conducted using neighbor-joining tree-building methods. There were five haplotypes （Ha to Hs） detected among the samples, and H5 was the main type. Phylogenetic analysis supported these observations. The haplotype diversity （H） and nucleotide diversity （Pi） were 0. 418 and 0. 000 66, respectively. It was demonstrated that the genetic homology of these isolates with the 12S gene of E. granulosus G1 genotype（AF297617）was over 99.86%. All 42 samples were iden- tified as E. granulosus sensu stricto （G1-G3 clusters）. In this study,genotypes H1 to H4 has notbeen identified elsewhere.

关 键 词：细粒棘球蚴 12S基因 遗传多态性 青海 

分 类 号：S852.734[农业科学—基础兽医学]