钙拮抗剂对大鼠腹部皮瓣抗氧化作用的研究  

Study of the antioxidation effect of calcium antagonist in rat abdominal skin flap

作　　者：杨国华[1] 李志海[2] 杨春珠[3] 孟庆刚[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院急诊创伤外科,150001 [2]哈尔滨医科大学第一临床医学院整形外科,150001 [3]哈尔滨医科大学第一临床医学院急诊内科,150001

出　　处：《中华手外科杂志》2000年第1期56-57,共2页Chinese Journal of Hand Surgery

摘　　要：目的　探讨钙离子拮抗剂对大鼠腹部皮瓣缺血再灌注损伤的保护作用。方法　建立大鼠腹壁下动脉岛状皮瓣缺血再灌注的模型 ,检测应用钙离子拮抗剂维拉帕米 (Verapamil ,Ver)和汉防己甲素(Tetrandcin ,Tet)前后 ,皮瓣脂肪组织中超氧化物歧化酶 (SOD)活性、谷胱甘肽过氧化酶 (GSH Px)活性和丙二醛 (MDA〕含量的变化。结果　Ver和Tet可以明显地提高皮瓣的SOD活性和GSH Px活性 ,并可显著降低MDA的含量。结论　钙离子拮抗剂能减轻大鼠腹部皮瓣缺血再灌注损伤的脂质过氧化作用。Objective To observe the protective effect of calcium antagonist on the ischemia reperfusion injury in rat abdominal skin flap.Methods 33 SD rats were randomized into three groups. The abdominal island flap based on the inferior epigastric vessels was used as the ischemia reperfusion model. In the control group, normal saline was given intraperitoneally for 3 consecutive days preoperatively, while Verapamil (Ver) and Tetrandcin (Tet) were given in the other two groups respectively. Samples from the flap were harvested during ischemia and reperfusion periods to measure the changes of superoxide dismutase (SOD) activity,glutathione peroxidase (GSH Px) activity and malonyl dialdehyde (MDA) content. Results SOD activity and GSH Px activity were much higher in the Ver and Tet groups than in the control group, while the MDA content was remarkably decreased in these two groups.Conclusions Calcium antagonist, Verapamil and Tetrandcin, plays an important role in oxygen free radical scavenging and in reducing lipid peroxidation.

关 键 词：再灌注损伤 钙通道阻滞药 腹部皮瓣 抗氧化 

分 类 号：R965[医药卫生—药理学]