新型吸管式药敏检测盒的应用及山东省禽源病原菌抗药性监测网络的构建  

Application of Straw Antibiotic Susceptibility Test Kit and Construction of Bacterial Resistance Monitoring Network for Intensive Poultry Farms in Shandong Province

作　　者：白华[1,2] 骆延波[1,2] 齐静[1,2] 胡明[1,2] 朱小玲[1,2] 王永军 苏红[1,2] 李靖冉[1,2] 李颢[1,2] 张秀美[1,2] 吴聪明[4] 刘玉庆[1,2] 

机构地区：[1]山东省动物疫病防治与繁育重点实验室,济南250100 [2]山东省农业科学院畜牧兽医研究所 [3]济南市动物疫病预防与控制中心 [4]中国农业大学动物医学院

出　　处：《家禽科学》2012年第5期7-11,共5页Poultry Science

基　　金：山东省科技攻关项目(2010GNC10952);公益性行业科技项目(200903055;201203040);山东省农业科学院畜牧兽医研究所青年基金

摘　　要：为了适应兽医临床的简陋条件和在分散的养殖场进行联合抗药性监测，按照药敏试验中试管稀释法原理研制灵敏、准确、稳定、操作简单、无需专门仪器设备的原位吸管式药敏检测盒，4-7h快速判断11种药物对目标病原菌敏感性，指导临床用药，并在肉鸡养殖中进行了应用；同时联合六和、中慧、正大、九联、民和、春雪、益生等省内大型养殖企业，借助企业的技术体系建立山东省动物源病原菌抗药性监测网络、数据库、菌种库，利用网络技术对抗药性数据进行收集、汇总，并实现网上查询，对了解跟踪山东省动物源病原茵抗药性发展规律具有重要参考价值。In order to adapt to primitive clinic veterinary conditions and to conduct joint monitoring resistance in the scattered farms,the Straw Antibiotic Susceptibility Test Kit was explored based on test- tube dilution method principle,which was in situ,sensitive,accurate,stable,easy to operate,no special equipment required,determine quickly in 4 ~7 hours the target pathogen sensitivity to 11 kinds of antibiotics for selection of clinical treatment,and was applicated in broiler breeding farms;Furthermore,we constructed the animal source pathogen resistance monitoring network in Shandong Province,and its databases,culture collections,based on network technology and the technology system of the large-scale farming enterprises,such as Liuhe,Zhonghui,Zhangda,J data can be collected,aggregated,and online inquiry development law of animal source pathogens resistant iulian,Minhe,Chunxue,Yisheng.The antibiotic resistance by the network for understanding and tracking the in Shandong province valuably：

关 键 词：吸管式药敏检测盒 原位药敏检测 病原菌 兽医临床 监测网 

分 类 号：S854.5[农业科学—临床兽医学]