不同入肝血流阻断方式对荷瘤小鼠肝功能影响的实验研究  

Effect of Different Hepatic Occlusion on Liver Function in Tumor-bearing Mice

作　　者：史斌[1] 李崇辉[1] 陈永卫[1] 葛新兰[1] 张爱群[1] 董家鸿[1] 

机构地区：[1]解放军总医院全军肝胆外科研究所,北京100853

出　　处：《现代生物医学进展》2012年第14期2601-2604,共4页Progress in Modern Biomedicine

基　　金：国家传染病防治科技重大专项资助(2008zx10002-26);北京市科技计划课题(Z101107050210013)

摘　　要：目的:探讨不同血流阻断方式对荷瘤小鼠肝细胞功能的影响。方法:选择昆明小鼠24只随机分为三组,正常对照组(Suspe-nded operation,SO)、肝门阻断组(Occlusion of the portal triad,OPT)、保留肝动脉持续阻断门静脉(Occlusion of portal vein,OPV)各8只。采用门静脉注射肿瘤的方法建立肝癌模型,建模后3天采用阻断范围为左外叶和中叶、阻断时间为60分钟的入肝血流阻断方式,复流后5天后,通过测量3组对肝脏的缺血再灌注损伤程度以及病理学变化来评价不同血流阻断方式对肝细胞功能影响的程度。结果:门静脉注射小鼠肝癌细胞8天后,对照组测量小鼠正常丙氨酸氨基转移酶(ALT)值为66.5±22.3 IU/L,OPT组值为276.3±80.5 IU/L,OPV组值为89.6±28.4 IU/L,两组比较有统计学差异(P<0.01);对照组测量小鼠正常天冬氨酸氨基转移酶(AST)值为301.3±126.7 IU/L,OPT组值为1126.4±285.5 IU/L,OPV组值为438.6±150.7 IU/L,两组比较有统计学差异(P<0.01),病理组织学OPV组肝细胞损伤程度明显较OPT组轻。结论:保留肝动脉持续阻断门静脉可以减轻荷瘤小鼠肝脏的缺血再灌注损伤。Objective： To investigate the effect of different hepatic vascular occlusion maneuvers on liver function in tumor-bearing mice.Methods： Twenty-four Kunming healthy mice were randomly divided into three groups,including SO（sham operation）（n=8）,group OPT（occlusion of portal triad）（n=8）and group OPV（occlusion of portal vein）（n=8）.Three days after an hepatocellular carcinoma model was established using portal vein injection,mice underwent either simultaneous clamping of both the portal portal vein and the hepatic artery or selective clamping of the portal vein to the median and left liver lobes for 60 minutes.Sham-operated mice served as controls.The ischemia-reperfusion（I/R）,pathological changes of liver tissue were evaluated.Results： The OPV group showed significantly lower ALT,AST value in the restoration of blood flow after five days than the OPT group.Hepatocytes in the OPV group were much less injured than in the OPT group in histopathology.Conclusion： Presevering hepatic artery flow during portal triad blood inflow occlusion reduces liver ischemia-reperfusion injury in tumor-bearing mice.

关 键 词：肝脏 血流阻断 缺血再灌注损伤 荷瘤小鼠 

分 类 号：R332[医药卫生—人体生理学] R322.61[医药卫生—基础医学]