HPLC同时测定参附汤中新乌头碱、乌头碱、次乌头碱含量的研究  被引量:6

Simultaneous Determination of Mesaconitine,Aconitine,Hypaconitine in ShenFu Decoction by HPLC

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作  者:姚静[1] 张春椿[1] 熊耀康[1] 

机构地区:[1]浙江中医药大学,杭州310053

出  处:《浙江中医药大学学报》2012年第5期554-557,共4页Journal of Zhejiang Chinese Medical University

基  金:浙江省自然科学基金重大项目(Z2091224)~~

摘  要:[目的]建立参附汤中新乌头碱、乌头碱、次乌头碱的含量测定方法。[方法]采用Hypersil BDS C18(4.6mm×250mm,5μm)色谱柱,柱温25℃,流动相为甲醇、0.1%三乙胺,梯度洗脱,流速:1.0mL.min-1,检测波长237nm。[结果]新乌头碱在0.005~0.5mg.mL-1范围内线性关系良好,r=0.9999,平均回收率98.49%,相对标准偏差(RSD)为2.31%;乌头碱在0.005~0.5mg.mL-1范围内线性关系良好,r=0.9999,平均回收率99.88%,RSD为1.57%;次乌头碱在0.005~0.5mg.mL-1范围内线性关系良好,r=0.9999,平均回收率99.67%,RSD为1.75%。[结论]该方法学考察符合定量要求,结果准确,可用于同时测定参附汤中新乌头碱、乌头碱、次乌头碱的含量,为参附汤的质量控制提供依据。[ Objective ] To establish a method for the determination of mesaconitine, aconitine, hypaconitine in ShenFu decoction. [ Method] A Hypersil BDS C18 column(4.6mm×250mm, 5μm) was used at 25℃, and the mobile phase was consisted of methanol and 0.1% triethylamine for gradient elution with the flow rate 1.0 mL .min ^-1. The detection wavelength was 237nm, [Result] The linearity ranges for mesaconitine, aconitine, hypaconifine were 0.0{)5-0.5 mg.mL-1 (r=0.9999), 0.005~0.5 mg.mL-1 (r=0.9999) and 0.005~0.5 mg-mL ^-1 (r=0.9999), respectively. The average recoveries were 98.49% with relative standard deviation(RSD) 2.31%, 99.88% with RSD 1.57%, 99.67% with RSD 1.75%. [Conclusion] The quantitative method was feasible and beneficial for the quahty control of ShenFu decoction.

关 键 词:参附汤 新乌头碱 乌头碱 次乌头碱 HPLC 

分 类 号:R282.71[医药卫生—中药学]

 

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