心肌细胞兴奋-收缩偶联芯片检测技术的研究进展  

Progress Regarding Chip Technologies in Detection of the Excitation-contraction Coupling of Cardiomyocytes

作　　者：周洁[1] 肖丽丹[2] 吴成雄[1] 胡宁[1] 王华[1] 王平[1] 

机构地区：[1]浙江大学生物医学工程与仪器科学学院,生物传感器国家专业实验室,生物医学工程教育部重点实验室,杭州310027 [2]香港理工大学医疗科技及资讯学系,香港999077

出　　处：《中国科学：生命科学》2012年第6期455-464,共10页Scientia Sinica(Vitae)

基　　金：国家自然科学基金(批准号:81027003);浙江省科技厅国际合作项目(批准号:2010C14006)资助

摘　　要：兴奋-收缩偶联是指肌细胞的电激动经过转导引起肌细胞收缩等一系列级联反应和信息传递过程.心肌细胞作为肌细胞的一种,它的兴奋-收缩偶联是心脏正常生理功能的基础.电兴奋和机械收缩作为ECC两个标志性的生理过程,其作用呈双向性,任何一阶段的异常都会导致心肌细胞ECC障碍,深入探讨和研究两者的相互作用为ECC异常导致的心律失常等心脏疾病的预防和治疗提供了新思路,寻求更为简便和有效的检测心肌细胞电兴奋和机械收缩的方法已成为研究者的一个热门研究课题.细胞芯片作为一种无损的实时传感检测手段,在生命科学、环境监测和药物开发等领域有着广泛的应用.本文首先概述了心肌细胞的ECC机制,接着针对国际上心肌细胞电兴奋和收缩检测领域最新研究成果,分析了多种芯片检测方法应用于心肌细胞ECC检测的可行性以及面临的问题,并介绍了一种实验室自主研发的多尺度电极阵列芯片.该芯片在不同电极位点分别记录细胞的电兴奋和机械收缩,可对ECC进行系统的分析,最后结合该芯片对芯片技术的应用前景进行了展望.Excitation-contraction coupling （ECC） denotes a series of cascades or signal transmissions caused by processes in which the excitation of myocytes stimulates the contraction through transduction, and it forms the basis of cardiac function at the cellular level. As two iconic physiological processes in ECC, the interaction of the excitation and contraction is bidirectional, which makes the abnormal of either one induce the defective ECC of cardiomyocytes. Studies on the interaction of the excitation and contraction provide new clues on the prevention and treatment of ECC abnormal-induced heart disease. Seeking for more convenient and effective methods in detecting the excitation and contraction of cardiomyocytes has been a hot topic among researchers. Cell chips, which serve as a noninvasion and real-time sensing technology, have been widely used in many fields, such as life science, environment monitoring and drug development. This paper first summarizes the ECC mechanism of cardiomyocytes, then describs the newest progress in monitoring either excitation or contraction. The feasibilities and defects of those chip detection methods are analyzed. We also introduce a chip with multi-scale electrode array carried out in our lab, which provides an in vitro platform to synchronously monitor excitation and contraction of cultured cardiomyocytes in different electrodes. It allows us to systematically analyze ECC. Finally, the prospect of chip technology is discussed.

关 键 词：兴奋-收缩偶联 心肌细胞 芯片技术 电兴奋 机械收缩 多尺度电极阵列 

分 类 号：R331[医药卫生—人体生理学]