不同灸量悬灸“大椎”穴对哮喘大鼠细胞免疫学机制的影响  

Effects of Different Quantity of Moxibustion at "Dazhui" (GV 14) on Cellular Immunity in Asthma Rats

作　　者：张伟[1] 陈明人[1] 熊俊[1] 

机构地区：[1]江西中医学院附属医院,南昌330006

出　　处：《针刺研究》2012年第3期202-205,217,共5页Acupuncture Research

基　　金：江西省教育厅青年科学基金项目(GJJ 11192)

摘　　要：目的:通过观察悬灸"大椎"穴不同灸量对哮喘大鼠血清白细胞介素4(IL-4)、γ干扰素(IFN-γ)、免疫球蛋白E(IgE)含量的影响,探讨艾灸治疗哮喘的细胞免疫调节机制以及最佳灸量。方法:SD雄性大鼠60只,随机分为空白对照组、哮喘模型组、治疗组1(悬灸15min)、治疗组2(悬灸30min)、治疗组3(悬灸60min)、治疗组4(悬灸120min)。哮喘模型采用腹腔注射致敏混悬剂并雾化吸入卵蛋白的方法制造。悬灸大鼠"大椎"穴,每日1次,共治疗7d。运用酶联免疫双抗体夹心法测定大鼠血清IL-4、IFN-γ、IgE含量,计算IFN-γ/IL-4比值。结果:模型组血清中IL-4、IFN-γ、IgE含量较空白组显著升高(P<0.01)。经过艾灸治疗后,各治疗组IL-4、IgE含量较模型组显著降低(P<0.01,P<0.05),IFN-γ/IL-4的比值显著升高(P<0.05,P<0.01);治疗组4IFN-γ含量较模型组显著升高(P<0.05)。治疗组2与治疗组1相比,IL-4、IgE含量显著降低(P<0.05),IFN-γ/IL-4显著升高(P<0.01);治疗组3与治疗组2相比,IL-4、IgE含量显著降低(P<0.01,P<0.05),IFN-γ/IL-4显著升高(P<0.01);治疗组4与治疗组3比较,各指标的变化不明显(P>0.05)。结论:悬灸"大椎"穴通过抑制血清IL-4的分泌,减轻IgE的炎性反应,提高IFN-γ/IL-4水平,从而改善哮喘细胞免疫机制;随着灸量的增加,对哮喘大鼠细胞免疫机制的改善作用也在增加,以艾灸1h效果最佳。Objective To observe the effects of different quantity of moxibustion of ＂Dazhui＂ （GV 14）on the levels of serum interleukin-4 （IL-4）, interferon-y）, （IFN-y）, and immunoglobulin E （IgE） in asthma rats, so as to reveal its mechanisms un- derlying immune regulation. Methods Sixty male SD rats were randomly divided into control, model, moxibustion-15 min（M- 15 min）, M-30 rain, M-60 m in, and M-120 min groups （10 rats/group）. Asthma model was established by intraperitoneal injection of sensitized-suspension （containing egg albumin, aluminium hydroxide, and deactivated Bacillus pertussis） and ovalbumin-aerosol inhalation. Moxibustion was applied to ＂Dazhui＂ （GV 14） for 15, 30, 60 and 120 min, respectively. Serum IL-4, IFN-y and IgE contents were assayed by ELISA （enzyme-linked immunosorbent assay）. Results Compared with the control group, serum IL-4, IFN-y and IgE levels were increased significantly（ P〈0. 01 ）, and serum IFN-y/IL-4 level was down-regulated obviously （P〈0.01）in the model group. After the moxibustion treatment, the levels of serum IL-4 and IgE in the M-15 min, M-30 min, M- 60 min, and M-120 min groups were reduced significantly compared with the model group（ P〈0. 01, P〈0. 05）. The ratios of IFN- y/IL-4 in the M-15 min, M-30 min, M-60 min, and M-120 min groups were significantly higher than that in the model group （P〈0. 05, P〈0. 01）. Comparison among the M-15 min, M-30 min, M-60 min, and M-120 min groups showed that the therapeutic effects of the M-30 min group were evidently superior to those of the M-15 min group, and those of the M-60 min group were significantly superior to those of the M-30 min group in lowering serum IL-4 and IgE contents and up-regulating IFN-y/IL-4 （P〈0. 05, P〈0. 01）. No significant differences were found between the M-60 min and M-120 min groups in down-regulating IL-4 and IgE levels, and in raising IFN-y/IL-4 level, as well as among the M-15 min, M-30 min, M-60 min and M-120 min groups in.elevating

关 键 词：哮喘 艾灸 "大椎"穴 灸量 白细胞介素4 Γ干扰素 免疫球蛋白E 细胞免疫 

分 类 号：R245.81[医药卫生—针灸推拿学]