电针对雌雄两性实验性肥胖大鼠脂代谢的影响  

Effect of Electroacupuncture on Lipid Metabolism in Male and Female Obesity Rats

作　　者：孙东云[1] 孙立虹[1] 梁玉磊[1] 张书义[2] 肖红玲[1] 吴中秋[1] 李文丽[3] 李新华[1] 马红利[1] 

机构地区：[1]河北医科大学中医学院针灸系,石家庄050091 [2]北京市丰台区兴隆中医院中医科,北京100141 [3]河北医科大学中医学院实验中心,石家庄050091

出　　处：《针刺研究》2012年第3期206-210,共5页Acupuncture Research

基　　金：河北省中医药管理局科学研究计划项目(No.2010002)

摘　　要：目的:探讨电针刺激对雌雄两性肥胖大鼠脂代谢影响的差异及其作用机制。方法:SD大鼠随机分为模型雌组、模型雄组、电针雌组、电针雄组,并设正常雌组和正常雄组进行对照。采用谷氨酸钠和高脂饮食诱导的下丘脑性肥胖模型。电针雌组、雄组电针大鼠"后三里""三阴交""丰隆"等穴。计算各组治疗前后Lee’s指数,生化法检测各组血浆甘油三酯(TG)、胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)含量。结果:电针雌、雄两组的Lee’s指数及血浆TG、TC、LDL-C含量均较模型雌、雄两组明显降低(P<0.01,P<0.05),而血浆HDL-C含量均有明显升高(P<0.05)。电针雄组Lee’s指数较电针雌组明显降低(P<0.05);电针雌组血浆TG、TC含量较电针雄组明显降低(P<0.05);电针雌组血浆HDL-C、LDL-C的含量与电针雄组比较差异无统计学意义(P>0.05)。结论:电针刺激对雌雄两性肥胖性大鼠均有不同程度的减肥作用,但在降低Lee’s指数和调节脂代谢方面存在着一定的差异,电针可更明显地降低雄性大鼠的Lee’s指数及雌性大鼠的血浆TG、TC含量,但对雌雄两性血浆HDL-C、LDL-C含量的影响无明显差异。Objective To investigate the influence of electroacupuncture （EA） on lipid metabolism in both sexes of obesity model rats. Methods A total of 30 male and 30 female SD rats were randomized into normal control, model and EA groups respectively, with 10 rats in each group. EA （2 Hz, 1-2 mA） was applied to the ipsilateral ＂Zusanli＂ （ST 36 ）-＂ Sanyinjiao＇（SP 6） and bilateral ＂Fenglong＂ （ST 40） for 30 min, once daily for 28 days. In addition,＂Quchi＇（LI 11 ） ,＂Zhongwan＂（CV 12） and＂Guanyuan＇（OV 4） were punctured with filiform needles and stimulated by manipulating the needle for 1 rain, separately. Obesity model was duplicated by subcutaneous injection of 15% glutamic sodium （0.2 mL/10 g） once daily for 5 days and by feeding the animal with high fat forage for 36 days. Lee＇s index was calculated, and plasma triglyeride （TG） concentration detected by glycerol-phosphoric acid oxidase-peroxydase method, total cholesterol （TO） detected by cholesterol oxidase method, and plasma low-density lipoprotein-cholesterol （LDL-O） and high-density lipoprotein-cholesterol （HDL-Q） contents were assayed by one-step method, respectively. Results In both male and female rats, compared to their own normal groups, the Lee＇s index, plasma TG, TO and LDL-C contents were increased significantly and plasma HDL-C contents decreased obviously in the model groups （P〈0.01）, while compared to their own model groups, the Lee＇s index, plasma TG, TC and LDL-C levels in the EA groups were down-regulated considerably and plasma HDL-C contents up-regulated remarkably after the treatment （P〈0.01, P〈0.05）. The efficacies of the EA intervention were obviously better in reducing Lee＇s index for male rats, and in down-regulating plasma TG and TO contents in female rats （ P〈0.05）. No significant differences were found between male and female rats in EA intervention for plasma LDL-O and HDL-O levels. Conclusion EA intervention can effectively regulate obesity ra

关 键 词：电针 肥胖 雌雄两性大鼠 Lee's指数 脂代谢 减重 

分 类 号：Q493.5[生物学—生理学]