苯致染色体损伤与X线修复交叉互补基因1多态关系的研究  

Polymorphism of XRCC1 and chromosome damage in workers occupationally exposed to benzene

作　　者：张静[1] 吕建萍[2] 张晨[3] 周莉芳[4] 叶云杰[4] 孙品[4] 程周祥[2] 夏昭林[4] 

机构地区：[1]新疆医科大学附属肿瘤医院科研科,乌鲁木齐830011 [2]安徽省芜湖市疾病预防控制中心 [3]新疆医科大学公共卫生学院毒理学教研室 [4]复旦大学公共卫生学院劳动卫生教研室

出　　处：《中华劳动卫生职业病杂志》2012年第6期423-427,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助（81001235）

摘　　要：目的探讨DNA损伤修复基因——X线修复交叉互补基因1（XRCC1）多态性与苯致外周血淋巴细胞染色体损伤的关系。方法采用胞质分裂阻滞微核试验方法评价459名苯接触工人和88名非接苯对照组工人染色体损伤水平，应用聚合酶链反应-限制性片段多态性技术检测XRCC1第6外显子194密码子，第9外显子280密码子和第10外显子399密码子多态。结果接苯组微核发生率（2．12‰±1．88‰）明显高于对照组（1．19‰±1．68‰），差异有统计学意义（P〈0．01）；〉35岁年龄组的接苯工人微核发生率（3．00‰±2．76‰）明显高于≤35岁年龄组（2．02‰±1．71‰），差异有统计学意义（P〈0．05）。未发现XRCC1基因多态性与微核率有关。与AAA／AAA野生型比较，携带AAA／BAA、AAB／AAB、ABA／ABA及ABB／ABB双体型的接苯工人微核率明显升高，差异有统计学意义（p〈0．05）。结论苯接触能致作业工人染色体损伤。年龄及XRCC1基因双体型与苯致染色体损伤有关。Objective To explore the relationship between the polymorphisms of DNA repair gene （XRCC1 194, 280 and 399） and the chromosomal damage induced by benzene. Methods The chromosomal damage of the peripheral lymphocytes in 459 workers occupationally exposed to benzene and 88 non-exposed controls were detected with cytokinesis-block micronucleus （CBMN） assay. PCR-RFLP technique was used to measure polymorphisms in XR CC1 194, 280 and 399. Results It was found that the MN frequency （2.12‰± 1.88‰） of the exposed group was significantly higher than that （1.19‰±1.68‰） of the control group （P〈0.05）, in the exposed group, the MN frequency （3.00‰±2.76‰） of older workers （〉35 years） was significantly higher than that （2.02‰±1.71‰） of younger workers （≤35 years） （P〈0.05）. The effect of genetic polymorphisms of XRCC1 on CBMN was not found. The haplotypes AAA/BAA, AAB/AAB, ABA/ABA, ABB/ABB could associated with the increased frequencies of total micronucleus （P〈0.05）. Conclusion Benzene exposure could result in chromosome damage. Age of workers and diplotypes of XRCC1 could associated with chromosomal damage induced by benzene.

关 键 词：苯 DNA损伤 微核试验 多态性 单核苷酸 

分 类 号：R131[医药卫生—劳动卫生]