葡萄籽原花青素对肾血管性高血压大鼠血管重塑的影响  

Effects of grape seed procyanidin on vascular remodeling in renovascular hypertensive rats

作　　者：吴秀香[1] 张妍[1] 孙柳青[1] 唐剑涛[2] 靳俊峰[1] 

机构地区：[1]遵义医学院珠海校区病理学与病理生理学教研室,广东珠海519041 [2]遵义医学院珠海校区计算机教研室,广东珠海519041

出　　处：《中国病理生理杂志》2012年第6期1023-1027,共5页Chinese Journal of Pathophysiology

基　　金：珠海市科技局资助项目(No.PC20071045);贵州省科技厅资助项目(No.黔科合J字[2008]2317);贵州省高层次人才科研条件特助经费资助项目(No.TZJF-2008年58号)

摘　　要：目的:观察葡萄籽原花青素(GSP)对肾血管性高血压(RH)大鼠血管重塑的影响并初步探讨其机制。方法:采用两肾一夹法建立RH大鼠模型,并设假手术组(对照组,n=7)。术后2周,选取鼠尾动脉收缩压升至130 mmHg以上的大鼠28只为RH大鼠,随机分为4组(n=7):RH模型组、GSP低剂量(50 mg.kg-1.d-1)治疗组、GSP高剂量(200 mg.kg-1.d-1)治疗组和卡托普利(30 mg.kg-1.d-1)阳性对照治疗组。治疗6周后,分别测定各组大鼠尾动脉收缩压、胸主动脉中膜厚度(MT)、管腔内径(LD)及MT/LD;Masson三色法观察各组胸主动脉胶原蛋白的含量;ELISA法测定主动脉中血管紧张素II(Ang II)含量;Western blotting法检测腹主动脉中TNF-α的蛋白表达。结果:治疗6周后,与对照组相比,RH模型组大鼠的尾动脉收缩压、MT、MT/LD、主动脉中的胶原含量、AngⅡ和TNF-α水平均明显升高,而LD则降低;与RH模型组相比,GSP能显著降低RH大鼠的尾动脉收缩压、主动脉MT、MT/LD,减少大鼠主动脉中胶原和AngⅡ含量,减少TNF-α的蛋白表达,使LD增大。高剂量GSP治疗组的作用尤为显著,与卡托普利治疗组的作用相当。结论:GSP不仅能显著降低RH大鼠尾动脉收缩压,而且对血管重塑有明显的逆转作用,其机制可能与降低主动脉中AngⅡ的含量和TNF-α的蛋白表达有关。AIM： To study the effect of grape seed procyanidin （GSP） on vascular remodeling in renovascular hypertensive （RH） rats. METHODS： The RH rat model was established by two-kidney one-clip method. Two weeks after operation, 28 rats were selected according to the increased tail systolic pressure above 130 mmHg and randomly divided into 4 groups （n=7）： RH model group, low GSP treatment group （50 mg·kg-1·d-1）,high GSP treatment group （200 mg·kg-1·d-1） and captopril treatment group （30 mg·kg-1·d-1）. Meanwhile, 7 rats with sham operation served as controls. Tail systolic pressure, medial thickness （MT）, luminal diameter （LD）, and the ratio of MT to LD in thoracic aortic wall were determined 6 weeks after treatment. Masson staining and ELISA were used to detect the content of collagen and angiotensin II （Ang II） in aortic tissues. The protein expression of tumor necrosis factor α （TNF-α） in abdominal aortic tissues was determined by Western blotting. RESULTS： Compared with control group, the tail systolic pressure, MT, MT/LD, the content of collagen and the protein expression of TNF-α in aorta were significantly increased in RH model group, but LD decreased. Treatment with GSP and captopril reduced the raised parameters, and increased the LD in RH model rats. These effects were more notable in high GSP treatment group, and equal to captopril treatment group. CONCLUSION： GSP treatment significantly decreases tail systolic pressure in RH rats, and effectively attenuates arterial vascular remodeling by decreasing the content of AngII and reducing the protein expression of TNF-α in aorta.

关 键 词：葡萄籽原花青素 肾血管性高血压 血管重塑 血管紧张素II 肿瘤坏死因子 

分 类 号：R544.1[医药卫生—心血管疾病] R282.71[医药卫生—内科学]