TEKT4蛋白在特发性弱精子症患者精子中的表达  

Expression of TEKT4 protein decreases in the ejaculated spermatozoa of idiopathic asthenozoospermic men

作　　者：武文斌[1] 李玉山[2] 吉晓菲[2] 王全先[2] 高学敏[2] 杨险峰[2] 潘周辉[2] 冯晓霞[2] 

机构地区：[1]郑州大学第三附属医院生殖中心,河南郑州450052 [2]郑州大学第三附属医院人类精子库,河南郑州450052

出　　处：《中华男科学杂志》2012年第6期514-517,共4页National Journal of Andrology

基　　金：郑州市科技攻关计划项目(112PPTSF318-7)~~

摘　　要：目的:探讨TEKT4蛋白在特发性弱精子症发生机制中的作用。方法:采用非连续密度梯度离心分离和纯化特发性弱精子症患者和正常男性精子,采用RT-PCR和Western印迹方法,从mRNA和蛋白水平检测TEKT4的表达及其差异。结果:RT-PCR结果表明,TEKT4 mRNA在特发性弱精子症患者精子中的表达水平与正常男性相比显著降低(0.59±0.13 vs 0.75±0.15,t=4.325,P<0.05);Western印迹结果与RT-PCR结果一致,TEKT4蛋白在特发性弱精子症患者精子中的表达水平与正常男性相比亦显著降低(0.48±0.14 vs 0.69±0.13,t=5.939,P<0.05)。结论:特发性弱精子症患者精子中TEKT4表达水平显著降低,可能是导致弱精子症发生的重要环节之一。Objective： To investigate the role of the TEKT4 protein in the pathogenesis of idiopathic asthenozoospermia. Methods： We separated and purified the ejaculated sperm from idiopathic asthenozoospermia patients and normozoospermic men by Percoll discontinuous density gradients, and detected the distribution and the expressions of TEKT4 mRNA and TEKT4 protein by RT- PCR and Western blot. Results ： RT-PCR revealed that the expression of TEKT4 mRNA was significantly lower in the sperm of the idiopathic asthenozoospermia patients than in those of the normozoospermic men （0.59±0.13 vs 0. 75 ±0.15, t = 4. 325, P 〈 0.05 ） , and Western blot confirmed the results of RT-PCR （0.48 ±0.14 vs 0. 69± 0.13, t = 5. 939, P 〈 0.05 ）. Conclusion ： The expres- sion of TEKT4 is significantly decreased in the ejaculated sperm of idiopathic asthenozoospermia patients, which might be one of the causes of idiopathic asthenozoospermia.

关 键 词：TEKT4 特发性弱精子症 逆转录-聚合酶链反应 免疫印迹 

分 类 号：R698.2[医药卫生—泌尿科学]