卵子玻璃化冷冻在体外受精-胚胎移植中的初步应用  

Vitrification of oocytes for in vitro fertilization and embryo transfer

作　　者：滕晓明[1] 李昆明[1] 王羽[1] 潘家坪[1] 尹萍[1] 梁珊珊[1] 阮井玲[1] 陈智勤[1] 

机构地区：[1]同济大学附属第一妇婴保健院生殖医学中心,上海200040

出　　处：《中华男科学杂志》2012年第6期531-533,共3页National Journal of Andrology

摘　　要：目的:探讨体外受精-胚胎移植(IVF-ET)治疗中的卵子冷冻保存临床应用的可行性及其指征和方法,以提高卵子利用率和减少卵子的废弃。方法:在我院生殖医学中心进行IVF-ET治疗的患者,在取卵日因男方因素无法进行受精者或获卵数超过25枚,进行知情同意后对部分卵子进行玻璃化冷冻保存。将冷冻保存卵子解冻后继续使用,或捐赠给需要受卵患者。结果:2009年10月至2011年6月,已有7例患者的卵子进行了复苏。共复苏卵子53枚,存活44枚,复苏率83.02%;有41枚MⅡ卵子行ICSI,受精32枚,受精率72.73%;形成30枚胚胎,卵裂率93.75%,获有效胚胎23枚。9个移植周期移植胚胎16枚,临床妊娠2例,分娩3个健康婴儿,胚胎种植率18.75%,活产率22.22%。剩余冷冻胚胎7枚。结论:卵子的玻璃化冷冻保存,可以获得比较满意的复苏率和受精率;将获卵较多的患者进行部分卵子的冷冻保存可以提高卵子的利用率,可以作为临床治疗的一个可选择的方法。Objective ： To explore the feasibility, indication and method of oocyte vitrification during the IVF - ET procedure, so as to increase the utilization of oocytes and reduce oocyte waste. Methods ： This study included the patients whose husbands failed to provide sperm samples at the time of oocyte pickup or from whom more than 25 oocytes were obtained. With the patients＇ consent, some of their oocytes were subjected to cryopreservation by vitrification, and used for IVF - ET after thawed. Results ： Totally, 53 oo- cytes from 7 patients were thawed, and 44 （83.02%） survived, of which 41 M lI oocytes were subjected to ICSI and 32 （72.73%） were fertilized. Thirty embryos were formed, with a cleavage rate of 93.75%. Sixteen embryos were transferred in 9 cycles, with a- chievement of 2 clinical pregnancies and delivery of 3 healthy babies. The implantation rate was 18.75% and the live birth rate 22. 22%. Seven of the embryos were still cryopreserved. Conclusion ： Cryopreservation of oocytes by vitrification effects satisfactory rates of survival and fertilization, and that of surplus oocytes can increase oocyte utilization and adds to the alternatives for IVF - ET.

关 键 词：卵子冷冻保存 玻璃化冷冻 体外受精-胚胎移植 卵细胞胞质内单精子注射 

分 类 号：R714.8[医药卫生—妇产科学]