卡介苗诱导小鼠脾脏树突状细胞分化作用研究  

BCG-induced mouse spleen DCs differentiation

作　　者：张淑芳[1] 方芳[2] 孙霞霞[3] 李莹莹[3] 台桂香[3] 

机构地区：[1]长春医学高等专科学校生化教研室,130031 [2]吉林医药学院免疫教研室,长春132013 [3]吉林大学白求恩医学院免疫教研室,长春130021

出　　处：《免疫学杂志》2012年第7期572-575,共4页Immunological Journal

基　　金：十二五科技部重大专项课题重大新药创制资助(2011ZX09102001-36);吉林省科技厅科技支撑计划重点项目(20080931)

摘　　要：目的探讨卡介苗(BCG)诱导小鼠脾脏树突状细胞分化作用。方法分别用PBS和BCG免疫小鼠,收集免疫鼠脾单核细胞培养,通过瑞士-姬姆萨染色观察脾单核细胞形态;流式细胞术分析脾巨噬细胞、树突状细胞表型;MTS法检测小鼠脾淋巴细胞增殖;ELISPOT法检测脾淋巴细胞IFN-γ的分泌;ELISA法测定小鼠脾单核细胞分泌IL-2情况。结果与对照组相比,BCG免疫组镜下可见体积大、带毛刺状突起的细胞较多;CD14(P<0.05)、CD40、CD11C、CD86、CD68表达水平增高;小鼠脾淋巴细胞刺激指数增高(P<0.05);分泌IFN-γ的细胞频数增多(P<0.01);脾单核细胞培养上清中IL-2水平明显增高(P<0.01)。结论 BCG可诱导小鼠树突状细胞分化并活化Th1细胞。To investigate inducing effects of BCG on mouse spleen DCs differentiation, we immunized female C57BL/6 mice with PBS and BCG, then harvested the mice spleens and isolated the splenic mononuclear cells by lymphocyte separation medium. We observed the cells modality by Switzerland-Giemsa staining, analyzed the splenic macrophages and DCs phenotype by flow cytometry, detected the splenic lymphocyte proliferation, IFN-γ-producing cells, and IL-2 secretion by MTS, ELISPOT, and ELISA, respectively. In BCG group, more large cells with burr bulge were observed under a microscope, and the expression levels of CD14 （P 〈 0.05）, CD40, CD11C, CD86, CD68 were increased. Compared with the PBS group, the lymphocyte stimulating index was higher, the number of IFN-γ producing cell was increased, and high level of IL-2 secretion was found in the BCG group （P 〈 0.05, P 〈 0.01, P 〈 0.01）. The results showed that BCG can induce DCs differentiation and active Thl cells in mouse.

关 键 词：卡介苗 树突状细胞 细胞分化 

分 类 号：R392.1[医药卫生—免疫学]