家兔缺氧后脑中血管活性肠肽含量的变化  

CHANGES IN REGIONAL CEREBRAL VASOACTIVE INTESTINAL PEPTIDE CONCENTRATION UNDER HYPOXIA IN RABBITS

作　　者：赵维民[1] 吕永达[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国应用生理学杂志》1990年第4期313-315,共3页Chinese Journal of Applied Physiology

摘　　要：本实验采用雄性青紫蓝家兔32只,随机分为急性缺氧组(模拟海拔5000m,2h)、低氧适应组(模拟海拔5000m,2周)和对照组。用放射免疫分析法分别测定了海平和模拟缺氧后大脑皮层、下丘脑和海马三个部位血管活性肠肽含量的变化。测定结果表明,急性缺氧组所测三个部位的血管活性肠肽含量较海平对照均有增高,其中下丘脑的含量自对照的12.1±1.1ng/g增至21.1±2.9ng/g(p<0.05),海马的含量自35.7±2.6ng/g增至45.9±1.7ng(p<0.01)。适应组三个部位的血管活性肠肽含量虽略高于对照,但均无统计学意义。这一结果与前人报道的在相应低氧条件下脑血流变化规律是吻合的。因此我们推测血管活性肠肽在缺氧条件下对脑血流的调节可能起重要作用。It had been found that vasoactive intestinal pcptide (VIP) could increase cerebral blood flow. The purpose of this study was to investigate the effect of hypoxia on the cerebral concentration of VIP. 32 male adult rabbits were divided into acute hypoxia (simulated altitude 5 000m, 2 hours), hypoxia-adapted (simulated altitude 5 000m, 2 weeks) and sea level control groups randomly. Radioimmunoassay method was employed to determine the regional concentration of VIP in cortex, hypothalamus and hippocampus. The results showed that in acute hypoxia group, VIP concentrations of the three areas were greater than those of control. In hypothalamus and hippocampus, the VIP content increased from 12.1 ± 1.1 to 21.1±2.9 ng/g tissue (p<0.05) and from 35.7± 2.5 to 45.9±1.7ng/g tissue (p<0.01) respectively. VIP concentrations in hypoxia-adapted group were slightly higher than control, but the differences were not statistically significant. It is suggested that VIP may play an important role in the regulation of CBF during hypoxia.

关 键 词：血管活性肠肽 缺氧 低氧适应 家兔 

分 类 号：R338[医药卫生—人体生理学]