抗体芯片在糖尿病肾病大鼠尿液细胞因子同步检测中的应用  

Analysis of urinary cytokines with antibody microarray in rats with diabetic nephropathy

作　　者：倪杰[1] 戴厚永[1] 郑敏[1] 吕林莉[1] 刘必成[1] 

机构地区：[1]东南大学附属中大医院肾脏病研究所,江苏南京210009

出　　处：《东南大学学报（医学版）》2012年第3期249-253,共5页Journal of Southeast University(Medical Science Edition)

基　　金：国家自然科学基金重点项目(81130010);国家重点基础研究项目(2012CB517700)

摘　　要：目的:应用抗体芯片技术同步检测糖尿病肾病(DN)大鼠尿液中多种细胞因子的水平,以初步探讨其在寻找DN生物标志物研究中的意义。方法:高糖高脂喂养联合低剂量链脲佐菌素(STZ)单次腹腔注射SHR大鼠诱导出2型DN模型,随机分为DN组(D组)和厄贝沙坦治疗组(T组,予厄贝沙坦50 mg.kg-1.d-1灌胃),并以同周龄WKY大鼠作对照(N组)。8周后应用RayBio大鼠细胞因子抗体芯片,同步检测各组大鼠24 h尿液中19种细胞因子水平的变化。结果:与N组相比,D组大鼠尿液中性粒细胞趋化因子(CINC)-3和Fractalkine细胞因子的水平明显升高。厄贝沙坦治疗后尿液CINC-3、Fractalkine、干扰素(IFN)-γ、白介素(IL)-1α、IL-10、脂多糖诱导的CXC趋化因子(LIX)和肿瘤坏死因子(TNF)-α共7种细胞因子水平显著性下降。T组与N组细胞因子水平差异无统计学意义。结论:首次将抗体芯片技术应用于DN模型的研究中,证实尿液中某些细胞因子水平的变化与DN的发生发展和转归密切相关,为DN新型生物标志物的发现提供了新的途径。Objective： To evaluate the influence of irbesartan on the urinary excretion of cytokines in rat with diabetic nephropathy by antibody microarray. Methods： 6-week old SHR were fed with high-lipids and high- sucrose diets and treated intraperitoneal injection with 35 mg. kg-l streptozotocin （STZ） to induce diabetic nephropathy. Then the rats were randomly divided into diabetic nephropathy group D and irbesartan-treatment group T （administered with irbesartan 50 mg-kg-1, d- 1 ）. Wistar-Kyoto （WKY） rats were used as controls （N）. At the end of 8 weeks, rat cytokine antibody array I（ RayBio） coated with 19 specific cytokine antibodies were probed with rat urine samples and the relative cytokine levels were compared among different groups. Results： Compared to the control rats, an increasing excretion of urinary neutrophil chemoattractant- 3 （ CINC- 3 ） and fractalkine was found in rats with diabetic nephropathy. After irbesartan administration, there was significant decrease in the levels of urinary cytokines including CINC-3,Fractalkine, IFN-T, IL-lc~, IL-10, lipopolysaccharide- induced CXC chemoattactant（LIX） and TNF-c~. No statistically significant changes were found between T group and N group. Conclusion： Antibody microarray was firstly applied in profiling urinary cytokine in DN animal models. A close correlation between certain urinary cytokine levels with the development of DN has been revealed, indicating a promising role of identifying urinary cytokines in diabetic nephropathy.

关 键 词：蛋白质阵列分析 糖尿病肾病 尿液 细胞因子 大鼠 

分 类 号：R33[医药卫生—人体生理学] R587.2[医药卫生—基础医学]