Pb^(2+)和Cd^(2+)对日本三角涡虫DNA损伤及损伤后修复的研究  

The Study of DNA Damage by Pb^(2+) and Cd^(2+) in Dugesia japonica and Damage Repair

作　　者：彭莹[1] 李亚男[1] 黄原[1] 叶海燕[1] 

机构地区：[1]陕西师范大学生命科学学院,西安710062

出　　处：《实验动物科学》2012年第2期1-4,共4页Laboratory Animal Science

基　　金：国家自然科学基金(No.30670279);陕西省自然科学基金(No.2007C101)

摘　　要：以日本三角涡虫(Dugesia japonica)为实验材料,采用急性毒性实验研究Pb2+、Cd2+胁迫下日本三角涡虫体细胞DNA损伤情况以及绿豆浸出液对DNA损伤的保护和修复机制。采用浓度为120 mg/L的Pb(NO3)2和1 mg/L的CdCl2溶液分别处理涡虫,紫外分光光度法和琼脂糖凝胶电泳检测24 h后三角涡虫DNA损伤情况。同时增加由绿豆浸出液进行修复的2组对照以研究绿豆对于DNA重金属损伤后的修复作用和效果。结果表明,Pb2+、Cd2+胁迫使日本三角涡虫DNA交联程度增加,并引起DNA链的断裂;绿豆浸出液对于由Cd2+胁迫引起的DNA损伤修复作用较好。而对于Pb2+胁迫,在绿豆浸出液与Pb2+同时培养的对照组中,推测该浸出液可能使Pb2+形成沉淀从而减小Pb2+浓度,因此使DNA损伤修复具有较好效果,对已经由Pb2+胁迫造成损伤的DNA,修复作用不大。We used technology of Ultraviolet Spectrophotometry and Agarose Gel Electrophoresis to analyze DNA damage under Pb^2＋ and Cd^2＋ stress in Dugesia japonica＇ somatic cell, and DNA damage repair by lixivium of green beans. The concentration of Pb^2＋ and Cd^2＋ were respectivelyl20 mg/L （Pb（ NO3 ） 2） and 1 mg/L （CdC12）. We also added control groups with lixivium of green beans to repair the damaged DNA. Planaria were exposed to 24 hours and extracted using method of Proteinase K . Though the analysis of experimental data. The results show that Lead and cadmium can induce DNA-DNA cross-linking and cut the DNA into smaller snippet. It had a better effect with the lixivium of green beans for damage repair caused by Cd^2＋ stress. For DNA damage caused by Pb^2＋ stress, it also had a better effect if we added the green beans lixivium at the same time with Pb （ NO3 ） 2. The reason is probable because the lixivium can precipitate Pb^2＋. But in control group which we added the lixivium after damage, there had not repairable effect in DNA damage.

关 键 词：DNA损伤 日本三角涡虫 Pb2+、Cd2+胁迫 绿豆浸出液 损伤修复 

分 类 号：Q95-33[生物学—动物学]