ISSR标记研究云南产牛奶菜属几种药用植物的亲缘关系  

Genetic relationship on several medicinal plants in Marsdenia from Yunnan in ISSR marker

作　　者：管志斌[1] 张丽霞[1] 宋美芳[1] 李海涛[1] 张忠廉[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所云南分所,云南景洪666100

出　　处：《中国中药杂志》2012年第11期1550-1552,共3页China Journal of Chinese Materia Medica

基　　金：中央级公益性科研院所基本科研业务专项(YZYN-10-02);西双版纳州三项经费专项(201107)

摘　　要：目的:分析常用傣药"傣百解"原植物等9种云南产牛奶菜属植物种间亲缘关系。方法:应用ISSR分子标记技术。结果:从60个ISSR引物中筛选出25个引物用于试验,共扩增出391个条带,每个引物检测到的位点为8～21,平均每个引物扩增出15.6个位点;种间遗传相似系数(GS)在0.667 5～0.821 0;9种牛奶菜属植物间,傣百解Marsdenia auricularis sp.nov.ined.为通光散M.tenacissima的近缘种,云南牛奶菜M.balansae与海枫屯M.officinalis间的亲缘关系最近。结论:ISSR结果支持傣百解为牛奶菜属植物,且与通光散近缘,牛奶菜属多种民族药和民间药值得深入研究。Objective： To analyze the genetic relationship of 9 Marsdenia species from Yunnan, especially the traditional Dai medicine ＂ Dai Bai Jie＂ （M. auricularis）. Method： Applying the inter-simple sequence repeats （ISSR） markers technology. Result： Twenty-five primers were screened out of 60 ISSR random primers and produced 391 bands totally, every primer produce 8-21 bands and the mean number was 15.6. The range of the GS（ genetic similarity） value was 0. 667 5-0. 821 0. In 9 Marsdenia species, M. au- ricularis is a relative of M. tenacissima. M. balansae and M. officinalis have the closest genetic relationship. Conclusion： It is suppor- ted by ISSR that the M. auricularis which is sib species of M. tenacissima, and the folk medicine of Marsdenia are worthy deep investi- gation and study.

关 键 词：牛奶菜 傣百解 ISSR 亲缘关系 

分 类 号：S567[农业科学—中草药栽培]