中国炭疽芽胞杆菌荚膜质粒基因单核苷酸多态性研究  

Study on the single nucleotide polymorphism in capsule plasmid gene of Bacillus anthracis in the China isolates

作　　者：张慧娟[1] 张恩民[1] 张建华[1] 魏建春[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所,传染病预防控制国家重点实验室 ,北京102206

出　　处：《中华流行病学杂志》2012年第6期593-596,共4页Chinese Journal of Epidemiology

基　　金：基金项目：国家科技重大专项（2008ZX10004-008）

摘　　要：目的研究中国炭疽芽胞杆菌（炭疽杆菌）荚膜质粒基因单核苷酸多态性（SNP）特征。方法选择中国不同分离年代、地点和来源的95株炭疽杆菌，采用PCR方法扩增荚膜质粒基因上的23个SNP位点，然后进行测序并进行聚类分析。结果通过聚类分析，95株炭疽杆菌可分为5个群，23个SNP位点中17个位点相同，6个位点存在多态性，其中17．89％（17／95）的菌株与参考菌株Pastuer同源，38．95％（37／95）的菌株与参考菌株AmesAncestor同源，其余菌株则不同于目前已知的全基因组测序菌株；3株菌在PS-34位点扩增基因片段中缺失长度约80bp的序列，待测的SNP位点包括在该缺失片段中；9株菌株在所有SNP位点扩增阴性，经炭疽杆菌荚膜质粒基因特异引物扩增证实这些菌株缺失荚膜质粒基因。结论中国炭疽杆菌荚膜质粒SNP位点具有遗传稳定性和自身的特异性，6个SNP位点可作为基因分型的指标。Objective To study the characteristic of single nucleotide polymorphism （SNP） in capsule plasmid gene of Bacillus anthracis isolated from China. Methods 95 Bacillus anthracis isolates from different sources were selected. 23 SNP sites were amplified by PCR method, sequenced and analyzed by clustering analysis. Results 95 Bacillus anthracis isolates were divided into 5 groups by cluster analysis. The identified isolates had the same sequence features in 17 sites and different nucleotide sequence in the other 6 sites of the 23 SNP sites. 17.89% （17/95） of the isolates had homologous locus sequences compared with the reference strain Pastuer. 38.95% （37/95） of the isolates had the homologous locus sequences compared with the reference strain Ames Ancestor. The remaining strains were different from those completed sequenced strains. 3 strains missed length of about 80 bp sequence in the PS-34 loci amplified gene fragment in which the tested SNP loci were included. 9 strains were amplified negative at all SNP loci and Bacillus anthracis capsule plasmid genes were missing which was confirmed by capsule plasmid gene-specific primers. Conclusion Results through analysis showed that single nucleotide genetic stability and specificity for capsule plasmid gene of Bacillus anthracis did exist in the Chinese isolates. The 6 discriminating SNP sites could be used as indicators in genotyping the Bacillus anthracis.

关 键 词：炭疽芽胞杆菌 单核苷酸多态性 基因分型 

分 类 号：R378[医药卫生—病原生物学]