过氧化物酶体增殖物激活受体的多个单核苷酸多态性与原发性高血压的关联研究  

Association between peroxisome proliferator-activated receptors gene polymorphism and essential hypertension

作　　者：蔺瑶[1] 顾淑君[1] 武鸣[2] 陈秋[3] 周正元[4] 俞浩[2] 张丽君[1] 骆文书[1] 郭志荣[1] 

机构地区：[1]苏州大学公共卫生学院,215123 [2]江苏省疾病预防控制中心 [3]苏州大学放射生物学教研室 [4]江苏省常熟市疾病预防控制中心

出　　处：《中华流行病学杂志》2012年第6期597-601,共5页Chinese Journal of Epidemiology

基　　金：基金项目：卫生部科学研究基金资助项目（WKJ2004-2-014）

摘　　要：目的探讨过氧化物酶体增殖物激活受体（PPAR）α／δ／γ10个单核苷酸多态性（SNP）位点与原发性高血压（EH）的关联。方法采用单纯随机抽样方法抽取“江苏省多代谢异常和代谢综合征综合防治研究（PMMJS）”队列人群中的820名研究对象，对其基线血样本的DNA进行PPARα／δ／γ10个SNP位点（PPARα：rs135539、rs1800206、r54253778；PPAR5：rs2016520、rs9794及PPARγ：rs10865710、rs1805192、rs4684847、rs709158、rs3856806）多态性检测。利用x2检验确定人群是否符合H．W遗传平衡定律，应用SHEsis软件对各位点进行连锁平衡检验，用logistic回归模型分析SNP与EH的关联。结果rs1800206、rs9794、rs10865710和rs4684847四个SNP位点在高血压和正常血压组间、高SBP和正常SBP组问及高DBP和正常DBP组间的分布差异有统计学意义（P〈0．05）。调整性别、年龄、体重指数、空腹血糖、高密度脂蛋白胆固醇和高脂饮食后，与野生型基因携带人群相比，rs1800206位点v等位基因携带人群发生高血压、高SBP及高DBP的OR值（95％C1）分别为0．60（0．41～O．89）、0．57（0．37～O．88）和0．61（0．39。0．96）；rs9794位点G等位基因携带人群发生高血压、高SBP及高DBP的OR值（95％CI）分别为0．63（0．46—0．87）、0．51（0．36～0．73）和0．68（0．47～1．01）；rs10865710位点G等位基因携带人群发生高血压、高SBP及高DBP的OR值（95％CI）分别为1．62（1．19～2．20）、1．59（1．14～2．22）和1．53（1．07～2．18）；rs4684847位点T等位基因携带人群发生高血压、高SBP及高DBP的OR值（95％C1）分别为1．42（1．04—1．94）、1．38（1．03—1．92）和1．37（1．00～1．88）。结论PPARa的rs1800206、PPARδ的rs9794及PPARγ的rs10865710和rs4684847四个SNP不同程度地影响高血压、高DBP及高SBP的发生。Objective To investigate the association between ten single nucleotide polymorphism （SNP） in the peroxisome proliferator-activated receptor （PPAR） α/δ/γand essential hypertension （EH）. Methods Participants were recruited within the framework of a cohort populations survey from the PMMJS （Prevention of Multiple Metabolic Disorders and MS in Jiangsu Province） which was conducted in the urban community of Jiangsu province from 1999 to 2007. Eight handred and twenty subjects （551 non-hypertensive subjects, 269 hypertensive subjects） were randomly selected but were not related to each other. Ten SNP （rs135539, rs1800206, rs4253778 of PPARa ; rs2016520, rs9794 of PPAR8 ; rs10865710, rs1805192, rs4684847, rs709158 and rs3856806 of PPART ） were selected from the HapMap database. X2 test was used to determine whether the whole population was in H-W genetic equilibrium. SHEsis software was used to examine the relations of SNP and linkageequilibrium. Logistic regression model was used to examine the association between ten SNP in the PPAR and EH. Results Difference on the distribution of four SNP genotypes including rs1800206, rs9794, rs10865710 and rs4684847 between high blood pressure and non-high blood pressure group, high systolic blood pressure （SBP） and normal SBP group, high diastolic blood pressure （DBP） and normal DBP group was significant （P〈0.05）. After adjusting factors as age, sex, body mass index, fasting plasma glucose, high density lipoprotein cholesterol-C, high-fat diet and compared with wild- type gene carriers, the OR （95% CI） of objects with rs 1800206 V allele appeared in high blood pressure, high SBP and high DBP were 0.60 （0.41-0.89）, 0.57 （0.37-0.88） and 0.61 （0.39-0.96）, respectively. The OR （95% CI） of objects with G allele of rs9794 were 0.63 （0.46-0.87）, 0.51 （0.36-0.73） and 0.68 （0.47-1.01）. The OR （95%CI） of objects with G allele of rsl0865710 were 1.62 （1.19-2.20） , 1.59 （1.14-2.22） and 1.53 （1.07-2.18）,

关 键 词：过氧化物酶体增殖物激活受体 单核苷酸多态性 原发性高血压 

分 类 号：R544.1[医药卫生—心血管疾病]