ABCB1基因多态性对卡马西平血药浓度的影响  

Effects of ABCB1polymorphisms on plasma carbamazepine concentration

作　　者：汝继玲[1] 何晓静[1] 邱枫[1] 孙亚欣[1] 肇丽梅[1] 朱旭[1] 

机构地区：[1]中国医科大学附属盛京医院药学部,辽宁沈阳110004

出　　处：《中国医院药学杂志》2012年第11期820-824,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金面上项目(编号:30973597);辽宁省科学计划项目(编号:2009225020)

摘　　要：目的:探讨ABCB1基因多态性对卡马西平血药浓度的影响。方法:采用荧光偏振免疫法(FPIA)测定275例口服卡马西平癫痫患者的血药浓度,聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP)或直接测序法检测ABCB1多态性位点C1236T、G2677T/A和C3435T的基因型。单因素方差分析计算各SNP位点不同基因型对应的卡马西平血药浓度是否有差异。结果:G2677T/A不同基因型对应的调整后血药浓度均值之间差别有显著性,TT基因型对应的调整后血药浓度显著高于GG型(P=0.001)。C1236T、C3435T各基因型对应的调整后血药浓度均值差别无统计学意义(P>0.05)。结论:ABCB1基因SNP位点G2677T/A的基因多态性对口服卡马西平癫痫患者的血药浓度有影响,提示TT基因型的患者可适当减少药物使用剂量。OBJECTIVE To investigate the effects of ABCB1 polymorphisms on plasma carbamazepine （CBZ） concentra tions. METHODS The plasma carbamazepine concentrations were determined by FPIA in 275 epileptic patients. C1236T, G2677T/A and C3435T polymorphisms of ABCB1 were genotyped by polymerase chain reaction amplification followed by restriction fragment length polymorphism analysis or by gene sequencing. CBZ concentrations of every genotype were compared u sing one-way ANOVA. RESULTS In G2677T/A, the adjusted CBZ concentrations of the patients with TT genotype were higher than those with GG genotype （P = 0. 001 ）. However, no significant difference was observed between the polymorphisms of C1236T and C3435T and the adjusted CBZ concentrations （P〉0.05）. CONCLUSION G2677T/A polymorphism of ABCB1 is associated with different plasma CBZ levels in patients with epilepsy. Our results suggest that the patients with 2677 TT genotype should properly reduce CBZ dose.

关 键 词：ABCB1 卡马西平 单核苷酸多态性 血药浓度 癫痫 

分 类 号：R969.3[医药卫生—药理学]