巴氯芬与吗啡联合应用对脊髓背角GABAB受体表达的影响  

The influence of GABAB receptor expression in the spinal dorsal horn of rats by using baclofen in combination with morphine

作　　者：单文燕[1] 陈艳平[2] 曹德权[2] 

机构地区：[1]湖南省人民医院麻醉科,长沙市410005 [2]中南大学湘雅二医院麻醉科

出　　处：《临床麻醉学杂志》2012年第5期491-493,共3页Journal of Clinical Anesthesiology

摘　　要：目的探讨巴氯芬与吗啡联合应用对脊髓背角GABAB受体表达的影响。方法成年雄性SD大鼠48只鞘内置管成功后,随机均分为四组,分别鞘内注射生理盐水10μl(NS组),吗啡10μg(M组),巴氯芬0.5μg(B组)和巴氯芬0.5μg+吗啡10μg(BM组)。每天9:00和16:00鞘内注射,在9:30行热水浴甩尾潜伏期(TFL)测定,连测3次,间隔5min,取其均值,将第1天注药后的TFL均值作为基础值,以TFL恢复到基础值作为出现吗啡耐受的标准。第11天晨,取大鼠腰段脊髓行免疫组织化学染色观察脊髓背角GABAB受体的表达。结果注药后第10天M组大鼠TFL恢复至基础值,出现吗啡耐受现象,B组和BM组未出现吗啡耐受现象(P<0.01)。M组GABABR1及GABABR2表达明显低于其它三组(P<0.01)。结论巴氯芬与吗啡联合应用可以减轻吗啡对脊髓背角GABAB受体表达的下调作用。Objective To investigate the effects of GABAB receptor expression in the spinal dorsal horn of rats by using baclofen in combination with morphine. Methods Healthy male SD rats were randomly divided into four groups after the success of intrathecal catheterization （n= 12）. They included saline group（group NS） ： 0. 90% saline 10 μl morphine group（group M） ： morphine 10μg; baclofen group（group B） .. baclofen 0.5 μg; baelofen-morphine group （group BM） ： baclofen 0. 5 μg ＋morphine 10 μg. Drugs were given by intratheeal injection on 9.. 00 am and 16.. 00 pm for 10 consecutive days. At 9：30 the tail-flick latency （TFL） in rats were measured continued 3 times with an interval of 5 min, The mean value of TFL measured on the first day were considered as the baseline, and the return to baseline level of TFL were regarded as the morphine tolerance standard. In the morning of the eleventh day, spinal lumbar enlargement of rats were removed and cut into frozen sections to test GABAB receptors expression by immunohistochemical staining. Results TFL of rats returned to baseline in group M after 10 days, the phenomenon of morphine tolerance occured; GABABR1 and GABAB R2 of group M in spinal dorsal horn were significantly lower than those of group NS,group B and group BM （P〈0.01）, The tolerance of group B and group BM didn＇t happen. Conclusion Baclofen in combination with morphine can reduce the down-regulated effect of morphine for the GABAB receptor in the spinal dorsal horn.

关 键 词：巴氯芬 吗啡耐受 GABAB受体 脊髓背角 

分 类 号：R96[医药卫生—药理学]