致奶牛乳腺炎大肠杆菌的分离与鉴定  

Isolationand identification of Escherichia coli isolated from mastitis cow

作　　者：徐继英[1] 刘俊林[1] 霍生东[1] 李宏胜[2] 曾宪辉[1] 陈化琦[2] 

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730030 [2]中国农业科学院兰州畜牧与兽药研究所

出　　处：《中兽医医药杂志》2012年第3期20-24,共5页Journal of Traditional Chinese Veterinary Medicine

基　　金：甘肃省自然科学基金项目(No.3ZS061-A25-072);西北民族大学基本科研业务费专项资金资助项目(No.zyz2011066)

摘　　要：从我国7个省(市、自治区)部分地区的奶牛场及养殖小区采集临床显性和隐性乳腺炎乳样病例530份,从中分离、鉴定出大肠杆菌95株,分离率为17.9%。通过玻板凝集和试管凝集试验结果表明,95个大肠杆菌分离株中,有54个分离株被鉴定出37种血清型,另有2株自凝,39株未定型,分别占检验菌株的56.85%、2.1%和41.05%。54个已定型菌株中,常见血清型为O93、O9、O146、O7、O74,分别有8、6、5、3、3株,分别占定型菌株的14.81%、11.11%、9.26%、5.55%、5.55%。且部分分离菌株可与多种单因子血清发生凝集反应,从而表现出多种血清型。To further investigate the prevalence of the pathogens caused bovine maslitis, totally 530 milk samples were collected from lactation cows with clinical and subclinical mastitis in different daily farms of seven provinces, autonomous regions and municipality in China from 2008 to 2009. Milk samples were submitted to Escherichia colt isolation and serotypes identification. The results showed that 95 （17.9%） E. colt isolates was obtained in 530 samples, in which 54 isolates were identified as 37 serogroups. 2 isolates were self-agglutinated, and 39 isolates could not be serotyped. O93, 09, O146, O7, and O74 serotypes were the most prevalent serogroups.

关 键 词：奶牛乳腺炎 大肠杆菌 血清型 

分 类 号：S853.74[农业科学—临床兽医学]