马尾松针叶聚戊烯醇对CCl_4致大鼠急性肝损伤的保护作用  

Protective effect of polyprenols from pine needles of Pinus massoniana on CCl_4-induced acute liver injury in rats

作　　者：郑光耀[1] 张良[2] 何玲[3] 薄采颖[1] 卞勇[2] 周维纯[1] 

机构地区：[1]中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室,江苏南京210042 [2]南京中医药大学药理学教研室,江苏南京210046 [3]中国药科大学药理学教研室,江苏南京210009

出　　处：《华西药学杂志》2012年第3期260-262,共3页West China Journal of Pharmaceutical Sciences

基　　金：科技部农业科技成果转化资金项目(2008GB24320412);2010年教育部"新世纪优秀人才"支持计划资助项目(NCET-10-0817);中央高校基本科研业务费专项资金项目(JKZ2009005)

摘　　要：目的观察马尾松针叶聚戊烯醇对CCl4所致大鼠急性肝损伤的保护作用。方法将SD大鼠随机均分为正常对照组、模型组、联苯双酯组(50 mg·kg-1)及聚戊烯醇低、中、高剂量组(10、20、40 mg·kg-1),连续7 d ig给予相应的药物后,ip含60%CCl4的橄榄油溶液,建立大鼠急性肝损伤模型,测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)的水平,在光镜下观察肝脏组织的病理学变化。结果马尾松针叶聚戊烯醇20、40 mg·kg-1剂量组能明显抑制急性肝损伤大鼠ALT、AST活性的升高,减轻CCl4引起的肝脏组织病理损伤。结论马尾松针叶聚戊烯醇对CCl4致大鼠急性肝损伤具有一定的保护作用。OBJECTIVE To investigate the protective effect of polyprenols from pine needles of Pinus massoniana on CCl4-induced acute liver injury of rats.METHODS SD rats were randomly divided into normal group,model group,bifendate group（50 mg·kg-1）,polyprenol low,middle and high-dose groups（10,20 and 40 mg·kg-1）.After the drugs were intragastric gavage for 7 d,all groups except the normal group were intra peritoneally injected with 60% CCl4 olive oil solution to induce acute liver injury model,and then the contents of AST and ALT in serum were measured and liver tissue pathology were also observed by optical microscope.RESULTS The polyprenols at the doses of 20 and 40 mg·kg-1 markedly decreased the activities of AST and ALT（P0.05,P0.01）,and reduced the damage of liver pathology.CONCLUSION The polyprenols from pine needles of Pinus massoniana has protective effects on CCl4-induced acute hepatic injury in rats.

关 键 词：松针 聚戊烯醇 CCL4 大鼠 急性肝损伤 保肝作用 

分 类 号：R96[医药卫生—药理学]