溪黄草黄酮类化合物抑制亚硝化反应活性的研究  

Study on the inhibition activities on nitrosation of flavonoids from Rabdosia serra

作　　者：段志芳[1,2] 付莉[2] 赵则海[2] 

机构地区：[1]肇庆学院生物医药工程中心,广东肇庆526061 [2]肇庆学院生命科学学院,广东肇庆526061

出　　处：《华西药学杂志》2012年第3期274-276,共3页West China Journal of Pharmaceutical Sciences

基　　金：肇庆学院青年项目(No.0927);森林植物生态学教育部重点实验室开放研究基金项目(No.KLP201101)

摘　　要：目的研究溪黄草黄酮类化合物对亚硝化反应的抑制作用。方法根据最佳提取工艺条件提取得到3个主要的黄酮类化合物,研究其对亚硝化反应的抑制作用。结果溪黄草总黄酮提取率可达91.98%,水溶性部分阻断亚硝胺的合成及清除亚硝酸盐作用较强,分离得到的3个黄酮类化合物中芦丁的抑制作用较强,对亚硝胺的合成的最大阻断率可达91.5%,对亚硝酸盐的最大清除率可达89.2%。结论溪黄草黄酮类化合物具有抑制亚硝化反应的活性。OBJECTIVE To study the nitrosation inhibition activities of flavonoids from Rabdosia serra Hara.METHODS The total flavonoids in Rabdosia serra were extracted using the optimum extraction conditions and were further separated to lipid-soluble and water-soluble parts by ethyl acetate.The structures of three flavonoids were identified and their inhibitions on nitrosation were studied.RESULTS The extraction rate of the flavonoids was as high as 91.98 %.The lipid-soluble and water-soluble extracts had some inhibition on nitrosation such as nitrosodimethylamine synthesis and sodium nitrite,in which the inhibition of the water-soluble extract was stronger than the lipid-soluble extract.In the three flavonoids,the rutin had stronger inhibition than the orthers,and its the maximum blocking rate to nitrosodimethylamine synthesis was as high as 91.5%,while the highest removal rate to sodium nitrite was 89.2%.CONCLUSION The flavonoids in Rabdosia serra Hara had inhibition on nitrosation.

关 键 词：溪黄草 黄酮 提取分离 抑制亚硝化 

分 类 号：R284.2[医药卫生—中药学]