金钮扣的薄层色谱鉴别及其绿原酸的测定  

Study on quality standard for Solanum torvum

作　　者：何洋[1] 刘建雄 周洪波[1] 李萍[1] 李慧珊[1] 房志坚[1] 

机构地区：[1]广东药学院中山校区,广东中山528458 [2]广东益和堂制药有限公司,广东中山528471

出　　处：《华西药学杂志》2012年第3期314-315,共2页West China Journal of Pharmaceutical Sciences

基　　金：中山市华南现代中药城(健康医药产业基地)专项发展基金(编号:2007J007)

摘　　要：目的薄层鉴别金钮扣药材并测定其中绿原酸的含量。方法采用TLC法定性鉴别,HPLC法测定绿原酸的含量。结果薄层色谱鉴别中,在与对照品色谱相应的位置上,供试品显相同的黄绿色荧光斑点。绿原酸1～60μg·mL-1与峰面积呈良好的线性关系(r=0.9999),平均回收率为99.6%,RSD=0.71%。结论所用方法准确可靠、灵敏度高、专属性强,可有效控制金钮扣药材的质量。OBJECTIVE To establish a method for quality control of Solanum torvum.METHODS TLC was used to qualitatively identify the main ingredients.HPLC was used to quantitatively determine chlorogenic acid content.RESULTS There was the same spot in colour and position between sample and reference in TLC.The HPLC calibration curve was linear within the range of 1-60 μg·mL-1（r=0.9999） for chlorogenic acid.The average recoveries were 99.6%（RSD=0.71%）.CONCLUSION The method is reliable,accurate and specific.It can be used for quality control of Solanum torvum.

关 键 词：金钮扣 薄层色谱法 高效液相色谱法 绿原酸 

分 类 号：R917[医药卫生—药物分析学]