铀矿尘致肺纤维化对PⅢNP、LN和TGF-β1影响  

Changes of PⅢNP,LN and TGF-β1 in plasma of rats with pulmonary fibrosis induced by uranium dust

作　　者：王守凤[1] 胡迎春[1] 袁新健[1] 杨丽萍[1] 宋凤梅[1] 贺性鹏[1] 

机构地区：[1]南华大学公共卫生学院预防医学系,湖南衡阳421001

出　　处：《中国公共卫生》2012年第6期802-804,共3页Chinese Journal of Public Health

基　　金：国家自然科学基金(30670634)

摘　　要：目的探讨铀矿尘致大鼠肺组织纤维化对血浆中Ⅲ型前胶原氨端肽(PⅢNP)、层粘连蛋白(LN)和转化生长因子-β1(TGF-β1)的影响。方法 60只大鼠随机分为铀矿尘组和对照组,铀矿尘组每只大鼠气管内一次性滴注20 mg/mL粉尘悬液1 mL,对照组给予等量生理盐水,分别于处理后第7、14、21、30及60 d采取肺组织及外周血,进行肺组织形态学观察及血浆中PⅢNP、LN和TGF-β1含量测定。结果在染尘后第21、30、60 d时,PⅢNP含量为(17.90±1.55)、(19.06±1.62)、(20.09±1.65)ng/mL,LN含量为(81.81±1.32)、(83.64±1.52)、(84.73±2.20)ng/mL,TGF-β1含量为(34.03±3.21)、(46.65±3.09)、(40.95±3.18)pg/mL,与对照组比较,差异有统计学意义(P<0.01),血浆中PⅢNP和LN含量增高与肺组织纤维化程度具有较好一致性。结论铀矿尘诱导大鼠肺组织纤维化过程中可引起血浆中PⅢNP、LN和TGF-β1含量明显增高。Objective To explore the changes of N-terminal propetide of type Ⅲ procollagen(PⅢNP),laminin(LN),and transforming growth factor-β1(TGF-β1) plasma in rats with pulmonary fibrosis induced by uranium dusts.Methods Sixty adult Wistar rats were divided randomly into control group(30 rats)and uranium dust group(30 rats).The intratracheal instillation method was used.Uranium dust group was exposed to 20mg/ml uranium dust suspension 1ml per rat,meanwhile control group was exposed to normal saline 1ml per rat.On the day of 7,14,21,30,and 60 after the exposure,6 rats in each group were killed and the peripheral blood samples were collected at same time.The levels of LN and PⅢNP in plasma were detected by radioimmunoassay and the level of TGF-β1 in plasma was measured with enzyme-linked immunosorbent assay.Results In the development of pulmonary fibrosis induced by uranium dust,the contents of PⅢNP,LN,and TGF-β1 in plasma were increased significantly at 21,30,and 60 days after the uranium dust exposure.On the day of 21,30,and 60 after the uranium exposure,the contents of PⅢNP were 17.90±1.55,19.06±1.62,and 20.09±1.65 ng/ml;the contents of LN were 81.81±1.32,83.64±1.52,and 84.73±2.20 ng/ml and the TGF-β1 were 34.03±3.21,46.65±3.09,and 40.95±3.18 pg/ml,respectively.The the contents of PⅢNP,LN,and TGF-β1 were significantly higher compared with those in the control group(P<0.01).The changes of PⅢNP and LN contents were associated with the development of pulmonary fibrosis.Conclusion In uranium dust-induced lung fibrosis of rats,the contents of PⅢNP,LN,and TGF-β1 in plasma increase significantly.

关 键 词：铀矿尘 肺纤维化 Ⅲ型前胶原氨端肽 层粘连蛋白 转化生长因子-β1 

分 类 号：R135.2[医药卫生—劳动卫生]